FastQCFastQC Report
Fri 29 Sept 2023
SRR12538978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12538978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6381435
Total Bases254.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4559587.14507003518801No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT4345936.8102707306428725No Hit
AGAAAAAAGTAGGTAAGGGAAGTCGGCNNNNNNNN2418093.789257431909907No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1875452.938915776780614No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAANN1799022.8191464772421875No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTANN1708032.67656099294281No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1545872.4224488692590302No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT1200861.8818024472552022No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAN1193011.869501138850431No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTTTAAGGAATTATAACAAAG800631.2546237640906786No Hit
ACTCTAGTCTGGCTTTGTGAAACGACTNNNNNNNN742411.1633903659600073No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTANNN705531.1055977221424336No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCAANNN693961.087467003894892No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT651771.0213533476404602No Hit
AGATTGTGCTGACTACGTCCCTGCCCTTTGTCTTAAGGAATTATAACAAA618190.96873195448986No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT575290.9015056958192005No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTNNNN355640.5573041173341107No Hit
CTTAAGGAATTATAACAAAGAAGTAACACGTNNNN351040.5500957073134805No Hit
GACTGTGAATCATCAGAATGCTGAATGTAAACNNN349900.5483092752648895No Hit
ACTAAAGATTGCCGTGTAGTAAGTATGTTNNNNNN339780.5324507732195031No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTNNNNN309420.4848752670833441No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTNNN302820.4745327657493965No Hit
AAGGACTGGAGAGCAGGTAGATGTGCATTATCCNN296470.46458202582961355No Hit
AGCTTTTTGATCCTTCGATGTCGGCTCTTCCTNNN284880.4464199666689389No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT273140.4280228506597654No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCANNN266420.41749230384701874No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTNN260830.40873251862629645No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTNNNNNNN256400.40179050636729824No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTNNN243200.38110550369940305No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGTNNNNNN242370.3798048558043763No Hit
AATTGTGCTCCAGGAACTTTGATGGCTTCTCNNNN239840.3758402302930297No Hit
GTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT233870.36648496772277706No Hit
ACGCGTGCTACACTGATATATATAACGAGTTATTTTTTGTTTTGTAGCAA230420.36107866020730445No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTANNN230050.36049885331434073No Hit
AGGACTGGAGAGCAGGTAGATGTGCATTATCCNNN228580.3581952961990524No Hit
TTTTACGATCTGTTGAGGCTTATCCCTAGTGGTNN210000.32907958789833325No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT188800.29585821997716816No Hit
AGTGTTGCGAAGCTAAGTCTGTTGGATNNNNNNNN182830.28650295740691556No Hit
AGCTTTTTGATCCTTCGATGTCGGCTCTTCCNNNN166640.2611324882256107No Hit
ATCCCCACTTTGCATACAATACCGGTAAGCANNNN158720.24872148662487356No Hit
AAATAGAAAAAAGTAGGTAAGGGAAGTCGGCNNNN133640.2094199815558726No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCTCNNN133010.2084327427921776No Hit
ATCAGATTGTGCTGACTACGTCCCTGCCCTTNNNN131720.20641125389508785No Hit
ATCAGACTACATTTGGAATTCTACTTTTATGTNNN128770.2017884692079446No Hit
AATCCCCACTTTGCATACAATACCGGTAAGCAAGGTGGGGAGTTTGACTG123230.19310703627005527No Hit
TGACTGTGAATCATCAGAATGCTGAATGTAAACNN117100.18350104639473722No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTAGNNN115540.18105645517034963No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTNN108500.17002445374747216No Hit
AAGAAGATTAAGAACACAATGTGAAAGAGCNNNNN108470.16997744237777238No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGNNNN107870.16903721498377716No Hit
AATCACGTTTAGGGGCGAAAGACTNNNNNNNNNNN105950.1660284873229924No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTANNNN104730.16411669162186873No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTT101310.1587573954760959No Hit
ACGATCTGTTGAGGCTTATCCCTAGTGGTNNNNNN95040.14893201920884566No Hit
AGGACCCGAGAGGCTTTGAACTAAGCGTGATGNNN93260.1461426779399931No Hit
TTAAGGAATTATAACAAAGAAGTAACACGTANNNN92510.14496739369749906No Hit
AACGGTGCCTTCTCAGAACTCTTGTCTTCACANNN92410.1448106891318332No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92250.1445599618267678No Hit
AGAACTGAAACGGACAAGGGGAATCCGACNNNNNN89730.1406110067719878No Hit
AAATCCCCACTTTGCATACAATACCGGTAAGCAAN88630.1388872565496632No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAANNNN88160.1381507450910336No Hit
TTTTACGATCTGTTGAGGCTTATCCCTANNNNNNN88140.13811940417790042No Hit
AGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCNN88060.13799404052536773No Hit
TGTTTTACGATCTGTTGAGGCTTATCCCTAGTGGTAAAGTGTTTT87490.13710082450107225No Hit
TTGAAGACTGAAGTGGAGAAGGGTTTCTTGTCNNN85380.13379435816552235No Hit
AATGAATTACTGTAGTTTACTTAAAGACGTNNNNN84980.13316753990285884No Hit
AACGATTGGTGCCGACTTTTTAACTAAAGAAANNN81860.12827835745408359No Hit
CCAAATCGTCTAGTGGTTAGGACTCCACGCTNNNN80260.12577108440342966No Hit
ATTAATGAATTACTGTAGTTTACTTAAAGACGTNN80040.12542633435896472No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTGGTNNNN79370.12437641376900337No Hit
AATAGATCCGACTTAGATAATGAAATTCCTANNNN78720.1233578340921752No Hit
TTAATGAATTACTGTAGTTTACTTAAAGACGTNNN78210.12255864080727924No Hit
ATTAGGCTGTGCGACTGGATCTGTTATGTCNNNNN74640.11696428781300758No Hit
ATTTCAATCACGTTTAGGGGCGAAAGACTNNNNNN74070.1160710717887121No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTNNNNNNN73170.11466073069771923No Hit
GACGTGGCATCTTTTCGGGCTAGTGAAGATTTNNN68730.10770304798215448No Hit
TTACGATCTGTTGAGGCTTATCCCTAGTNNNNNNN65830.10315861557784417No Hit
AGACGACTTTTTTGCCTCAGGGTGCTGTAAACANN65020.10188930859595059No Hit
TTTTTGATCCTTCGATGTCGGCTCTTCCTNNNNNN64620.10126249033328712No Hit
TTTACGATCTGTTGAGGCTTATCCCTAGNNNNNNN64450.10099609257165512No Hit
TTGTTTTACGATCTGTTGAGGCTTATCCCTNNNNN64310.1007767061797229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)