FastQCFastQC Report
Wed 28 Jun 2023
SRR1257226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40956566
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA28579066.977894582275282No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC25113076.131634668785464No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA10960712.6761789550422757No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA8539082.084911122675666No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC6613411.6147374269610395No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6236601.522735084772488No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT6053571.4780462795635747No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA5218041.274042359899021No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4328221.0567829343895676No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA4319301.0546050174226032No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4196371.0245902940202556No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3797140.9271138600828985No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3389130.8274936917318704No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC3292810.8039760950661733No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2503980.6113744985358393No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2474800.6042498777851639No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2282870.5573880388311853No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG2219570.5419326415207759No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1891340.46179164532495226No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1873630.457467552333367No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1849160.45149293033991184No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1842200.4497935691190516No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1648400.40247514891751424No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1606580.3922643319266562No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1482800.3620420715935999No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA1424620.34783677908934063No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1371220.33479857661894796No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1350210.3296687520140238No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1259360.3074867165377097No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1221530.29825010231570687No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1195430.2918774977374812No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA1193420.29138673393662934No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1094430.2672172271474127No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1074970.2624658522396629No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG1070230.2613085286495943No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1017990.248553553049345No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA992910.2424299927879696No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA962930.2351100431613334No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA950810.23215081069052518No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA912350.22276037497870305No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT836350.2042041317624139No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT831900.20311761488988117No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT809770.1977143298586117No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA797120.19462569200747934No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT772750.18867548612351923No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA762650.1862094590645124No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT759800.18551359994390154No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC759740.18549895027820448No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA726230.17731711198639066No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT705100.17215798805007235No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA638850.15598231550955713No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG632790.15450269927415303No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT632180.1543537610062328No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA619270.15120164127041313No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA612940.14965610153937223No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC597920.1459888018932056No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT542160.13237437923872816No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG514920.1257234310122582No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA498630.12174604677550359No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG490330.11971950968740885No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA484420.11827651761624743No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG475670.11614010803542466No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG472990.1154857563009555No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA471660.11516102204467045No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA467070.11404032261884454No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT443350.10824882144660274No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT441000.10767504287346748No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC440780.10762132743257821No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT439620.10733810056243484No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG439020.10719160390546414No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA434650.10612461992052752No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT432370.10556793262403884No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC430000.10498927082900456No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA426930.10423969626750446No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA425590.1039125204002699No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA421910.10301400757084955No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG418430.1021643269604195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)