FastQCFastQC Report
Sun 2 Jul 2023
SRR1257230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50014831
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC28823695.763028570465428No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA19774233.9536732614371926No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA9804061.9602305564123572No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG9669101.9332465604052527No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA8841751.7678256275623523No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT7438901.487338825557563No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA6489601.2975351251311835No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA6231891.2460084089857266No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG6197821.2391964295550653No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4754820.9506820087025787No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG4747210.9491604600243475No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC4718360.9433921710142337No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC4349310.869604058044303No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4106930.8211424327316033No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3366980.6731963165085972No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2918950.5836168875588122No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2828840.5656002316592852No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2688850.5376105339634157No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA2674580.5347573802658655No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC2568780.5136036548838884No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2510360.5019231195642748No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2339570.4677752485057882No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2085940.41706429039018444No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1940080.3879009408229331No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1841220.36813480385448066No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1782320.35635829700194327No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1576730.31525248980647363No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA1545090.3089263662612396No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1505740.30105869996841533No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1446130.289140235223428No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1438620.2876386806145561No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG1436470.2872088081233345No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1379730.2758641731689546No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1357320.27138350222556984No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1303760.26067467867681093No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT1287360.25739565130191083No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1287000.2573236726522179No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1255560.25103753724570216No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1149770.22988581127066088No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1145150.22896208526626832No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1110460.22202614260558035No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1108540.22164225647388472No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1062070.21235101244268922No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1054720.21088144834479197No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1031240.20618684085926434No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1013820.20270387397690096No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1010860.20211204952387024No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT992100.1983611621120943No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC962020.192346946048863No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC955630.1910693250168135No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA900360.18001860288201316No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA888040.17755533353696626No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT866770.17330259498427578No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG856470.17124320584028366No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGG810960.162143904874936No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCG797320.15941671381434838No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG792780.15850898306544314No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG790650.1580831093880933No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA771190.15419226349080334No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG765610.15307659442056298No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT754640.1508832450118646No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC746100.14917575148859344No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA746000.1491557574192343No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG728010.1455588243415238No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA722370.14443115882966795No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG719540.1438653266668041No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA716610.1432795004345811No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT700120.1399824783972578No Hit
AATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG699850.1399284944099881No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT693000.13855890065888654No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT692720.13850291726468095No Hit
CATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG644070.1287758025214561No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT635490.1270603113704413No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA628920.12574670101354538No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT628350.12563273481819823No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTTGTAGGCACCATC584010.11676736446435258No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCA583720.11670938166321106No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT571990.11436407732738314No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG562670.11250063006311069No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG544920.10895168275186214No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA536500.10726818211182199No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA534180.10680431970268979No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT533090.10658638434667508No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA518880.10374522709074034No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA510770.10212370806571354No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA503830.10073611965218877No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT501520.10027425664999247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)