FastQCFastQC Report
Wed 28 Jun 2023
SRR1257237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56071156
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC27586584.919923534303448No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA26179854.669040531284926No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA16327942.9120034550384517No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA11755582.0965467521304535No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT8649981.5426790915457496No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA8488151.5138175499716824No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA6850621.2217725634192382No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6536541.1657580236084308No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA5610871.0006695777772086No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG5394510.9620828933863963No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA4772040.8510685957678489No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4491050.8009554859186424No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4411020.7866825502937732No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT4147790.739736844376813No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA4144580.7391643575174374No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC3978790.709596570471991No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA3761330.6708137067835734No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA3727260.6647374989022877No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA3337240.5951794537640708No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3141130.560204251897357No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2727430.4864230015161449No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2689050.4795781274778783No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2617650.46684430761513107No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2468760.44029054796016687No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2390670.4263636012783471No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA2378720.424232380727089No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2193470.39119400356218803No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2185510.38977437882678934No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC2033000.3625750109378876No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1891090.3372660980986374No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1728280.30822977860488554No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1706580.30435969609758No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1607410.28667324069437766No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1591950.2839160298389425No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1566980.27946275978330104No Hit
GCTGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1435080.25593907855225956No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1409120.2513092471287733No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1363380.24315175524471083No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1353850.24145212914818448No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1342660.23945645065709006No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA1333010.23773542318264312No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1266940.2259521811892018No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA1266280.2258344736106386No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1259750.22466988196212684No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1244010.2218627345582103No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1213510.21642321767006192No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT1207390.21533174739611216No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1094560.19520910180628342No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1093470.19501470595683812No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA1063000.18958053941316994No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1038000.18512191901304834No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1015230.18106100755261761No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT998300.17804162981765526No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA981580.17505970449405395No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC953940.17013025377967952No Hit
TCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC951200.1696415889838262No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG940650.1677600511749749No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG920830.1642252569217585No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG907370.16182473569833303No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA851700.15189627979134226No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT832600.14848989380564936No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA815830.14549905124124782No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT795090.14180017975730697No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA792090.14126514530929238No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA789650.14082998395824048No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT767540.13688678007637295No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT757690.13513008363872503No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA755320.13470740642479354No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT744380.1327563141377003No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA731820.13051630324867924No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA731710.1304966853189187No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA715630.1276289006775605No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT690640.12317206372559894No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT686870.12249970376926061No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAG682850.12178275760892107No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG671570.11977102808438621No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA662930.1182301288741042No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA640300.11419418568791413No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT633730.11302246024676219No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA631870.11269073888899313No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG623960.11128003139439466No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAG623780.11124792932751376No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC613600.10943237910058426No Hit
TTCGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA611960.1091398936023363No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG608290.10848536812759844No Hit
TGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA605820.10804485643206643No Hit
TTATGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC603970.10771491852245743No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT601250.1072298206229242No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA588630.10497910904494281No Hit
CTGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT577090.10292100986824669No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA575810.10269272850376047No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCCTGTAGGCACCATC564860.10073985276850721No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT563230.10044915071841928No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC562180.10026188866161419No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)