FastQCFastQC Report
Wed 28 Jun 2023
SRR1257238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36688749
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC22355716.093342130580686No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA10397692.8340268565712066No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA7824852.132765551640913No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA7703562.0997063704734114No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA7222461.9685762520820758No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4159821.1338135295918648No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3690671.0059405405182935No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA3608250.9834758879350178No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3027720.8252448182411453No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA2561970.6982985437851805No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2524620.6881183111476491No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2483020.6767796852381094No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2354810.6418343672606552No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1991540.5428203616318452No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG1827850.4982045040565433No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1824960.49741679663157773No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1781530.4855793802072674No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1711570.46651086413439713No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1639860.44696536259658237No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC1548830.4221539415257795No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1468260.400193530719731No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA1394020.3799584444811678No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1300620.35450104880926847No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT1282530.3495703819173556No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC1185270.32306089259135No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1145830.3123110030271133No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1138370.3102776821308353No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1090240.2971592190292452No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1089770.2970311143615172No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC997790.2719607583240301No Hit
TCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC977930.26654765470471614No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG915690.24958332594005864No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA885860.24145276798617474No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC849390.2315123908967297No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA843290.22984975584749429No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT830770.22643726554971935No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA809290.22058260967142818No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT778700.2122449037441969No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT755680.20597050065675448No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG748580.20403530248469362No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC745610.20322579000990196No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA742740.20244353384739283No Hit
TTGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA737030.2008871984160594No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAG735680.20051923820024498No Hit
TGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA733920.20003952710407213No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA678130.18483323048163894No Hit
TTCGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA628300.17125141007124556No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA627510.17103608520421343No Hit
TTATGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC614820.16757725917555816No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA608400.1658274039270186No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA604030.1646363030802713No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT594690.16209056351308135No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA579030.15782222500963441No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG570930.15561446371474807No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT546510.14895847225535No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG546350.14891486215569794No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA544880.14851419436514446No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA543980.14826888755460155No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT536010.1460965594656825No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA533320.145363364665282No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA528390.1440196284697524No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA524230.14288576587879842No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG512910.13980035132841406No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA512260.1396231852985775No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATA511540.13942693985014315No Hit
TATTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC491660.13400838496837275No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA482140.13141358403907422No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT457620.12473033626739358No Hit
TCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA446540.12171033686648733No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA445590.12145140189980314No Hit
TCGAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG445360.12138871238155326No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT440180.1199768354053173No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA437050.11912371283087357No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA436580.1189956081631456No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA422130.11505707103831748No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA414900.11308643966028932No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACC411610.1121897069861935No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC411320.11211066368057411No Hit
TTTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG404610.11028176512641519No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA404280.11019181929588279No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA399720.1089489314557986No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG399470.10888079067509224No Hit
CTTAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG396240.10800041178836597No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAAT385350.10503219938079655No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT384740.10486593587587302No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA379310.10338591811893069No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC376230.10254642370062822No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA372930.10164696539530416No Hit
ATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGA370880.10108821099351195No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG370410.10096010632578396No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT369360.10067391504681722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)