FastQCFastQC Report
Wed 28 Jun 2023
SRR1257244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65054967
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC39085046.0080024327735035No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA30495504.687651290331144No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA14066312.162219219940577No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA12587721.9349360364751242No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA7744561.1904640578789318No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC6758861.0389460346663462No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA6314660.9706653144563119No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA5846180.8986523657755449No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT5443630.8367739238112287No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4833610.7430039892265259No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA4729500.7270005993546965No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4457830.6852405289822067No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4423780.6800064935856474No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG4123880.6339070158931908No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA3400240.5226718507135666No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3377490.5191748079743088No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA3095020.4757546030266989No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC2801050.4305666621889148No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2750730.4228316647981698No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2716120.4175115483495672No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2654660.4080641528878187No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2628500.4040429380280833No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT2601870.3999494765710972No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2600960.3998095948615269No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2482530.38160498951601957No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2324360.3572917037987276No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT2295080.3527908945061797No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2266890.3484576358327873No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2238610.3441105427046024No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2089960.321260634872046No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA2061640.3169073930972865No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1936610.29768826106698354No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1759040.27039288176104986No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1679000.2580894399654372No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1529740.23514576527261938No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT1425800.21916850714873162No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1378880.21195614471682078No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA1378290.21186545217984662No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1370900.21072948972520422No Hit
TCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1353850.20810862912281547No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1321770.2031774145700512No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1320630.2030021781426774No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1307290.2009516045100753No Hit
GCTGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1264070.19430799188630749No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1223060.1880040919857818No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1212460.18637470064353426No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA1154950.1775344840310195No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1140700.17534402868884708No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1078830.16583360959970972No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1075250.16528330573129027No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1049200.16127899964963477No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1042200.16020298649909392No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1037820.1595297096991841No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1037630.1595005036279551No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT993320.15268934038503162No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA989890.15216209394126662No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT977540.15026369931138386No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA973190.14959503399640492No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC953270.14653300800229443No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAA943570.14504196120797355No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG939360.1443948161560054No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT935740.14383836364101146No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA916100.1408193781729226No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC914580.14058572960309088No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA914480.14057035798665457No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA903510.13888409166359272No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA898320.1380863047705489No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT881390.1354838901078837No Hit
TTATGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC875380.1345600559600622No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT847810.13032210130857494No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT825760.12693265988437133No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA798360.12272083698082577No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAAT796110.12237497561100906No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA784930.12065642889343099No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG761100.11699337269666127No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA749990.1152855861105886No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT741530.11398514736007782No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA736350.11318889762867759No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT720590.1107663308783171No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT719500.11059878025916146No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT713180.10962729410038746No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA709390.10904470983745178No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAG709190.10901396660457915No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA687470.10567525151461532No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA669920.1029775328300451No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG654820.10065641874816415No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC652260.10026290536739493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)