FastQCFastQC Report
Wed 28 Jun 2023
SRR1257246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64758993
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC36758995.676275725905744No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA35461095.475855685402643No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA11783401.8195773983082162No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA10980801.695640943644692No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC8296681.2811626023894473No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA6275060.9689866548727835No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA5384090.8314042190248387No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT5296090.8178153727622046No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA5114310.7897451401074134No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA5089200.7858676863613366No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC4155900.6417487066236499No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4070450.6285536280652171No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4036170.6232601547710911No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC4007150.6187789238785724No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT3934650.6075835675826522No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA3385070.5227181342983513No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG3252970.5023194230336472No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2879250.44461006365556055No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2849040.4399450745010813No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA2844480.4392409251947448No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2746390.4240939941731336No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2718580.4197996099167262No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2491960.38480524241629266No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2462910.38031937896254814No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2314700.3574329823195367No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2279850.3520514903621185No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2196570.3391915003990257No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2106080.325218151554642No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2064250.3187588170186649No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1896180.29280566484410897No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT1862480.28760175440035024No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1841570.28437285922589933No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1823650.28160567598696296No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1649230.25467196501959194No Hit
TCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1630470.2517750700663304No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1491970.23038807907343464No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1414900.21848702928410269No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1394070.21527048760625417No Hit
GCTGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1379510.21302215122461832No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1310400.20235027434722463No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT1302730.20116588286047005No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1282400.19802655053638651No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1267140.19567012105947973No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1216010.18777469254347423No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1215300.18766505526112798No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1179110.1820766422356197No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1129550.1744236510904362No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1115340.17222936125643584No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1110290.17144954678340968No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1099730.1698188852318936No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA1078180.1664911620846235No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1045880.16150343783140667No Hit
TTATGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1027430.1586544126774794No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1005970.15534058721388702No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT982880.1517750592570209No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC980390.15139055667527135No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT973430.15031580247086299No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG949810.14666843259900597No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT944410.14583457157834434No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC923530.14261030896511934No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG921460.14229066224053238No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA907110.14007475378747783No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT903580.13952965574989715No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAA866420.13379145657808483No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA854220.13190754834621965No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA842830.13014871926745372No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA837120.1292669884474578No Hit
TATTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC823950.12723329406928857No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA820380.12668201928340672No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT804460.12422367345953017No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC792840.12242932807803235No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT759210.11723622694380068No Hit
TGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA755450.11665561260348814No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG698870.1079186021314445No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT690310.10659677799498828No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCA688900.10637904761737108No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA684340.10567489831103456No Hit
ATTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA683110.10548496330077277No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA677350.10459551154540034No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG676610.10448124170182818No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT674420.10414306473233764No Hit
TTCGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA668410.10321500830008275No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT667080.10300963141906792No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA663100.10239504496309879No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA658970.10175729570100016No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT651860.10065937869046233No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA651660.10062849494895637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)