FastQCFastQC Report
Tue 27 Jun 2023
SRR1257247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60896904
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA28182604.627920000662103No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC26073634.281601902126256No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA10298191.6910859704788932No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA8550681.4041239272196826No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA7070401.1610442461902497No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA6502481.0677849895291884No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC6134851.0074157464556819No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT5461500.8968436227890995No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4908600.8060508297761738No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA4762580.7820725992901052No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4581760.7523797925753336No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4575170.7512976357550131No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA4360480.7160429699348919No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG3887590.6383887758891651No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3792270.6227360918052584No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC3388890.5564962711404836No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA3278350.5383442810163223No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2569440.4219327800309848No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG2426120.39839792183852235No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2417670.39701033077149545No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2297480.3772736952275932No Hit
TTGTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2264060.37178573150451133No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT2256060.370472035819752No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA2167540.3559359930678906No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1986210.32615943825321564No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1858890.3052519714302717No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1774220.29134814472670073No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1756950.2885122041672266No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1755500.288274096824364No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1713440.2813673417617421No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1634170.2683502596453836No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1628410.26740439875235694No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1607000.2638886206760199No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1585350.26033343172914014No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1522760.2500554051155047No Hit
TTCTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1502770.2467728080232125No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1483070.24353783239949275No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA1333820.21902919728070247No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT1316110.2161210034585666No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1276980.20969538944048782No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1232780.2024372207821928No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC1216820.19981639789109804No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA1140390.1872656777428291No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1110560.18236723495828294No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1106770.18174487162762823No Hit
TTTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1075620.17662966905509678No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1052560.17284294124377816No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1046090.1717804898587291No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1024670.16826306966278615No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA999690.1641610548871253No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT953880.1566385049722725No Hit
GCTGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA943320.15490442666839022No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA933140.15323274890953406No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT932690.15315885352726633No Hit
TCTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC911160.14962337001565795No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA910590.14952976919811883No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA898770.147588783823887No Hit
TTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC870540.1429530801762927No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAA867250.14241282282593545No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT854200.1402698567401719No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG835440.13718924035941138No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA831000.13646013925436998No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA824490.1353911193908971No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG811150.13320053183656103No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA765510.12570589795500933No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT763400.12535941071815407No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA757860.12444967645645827No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC728540.11963498177181553No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC708270.11630640533055671No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG704740.11572673710965667No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG687510.11289736502860638No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA686520.11273479518761742No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA686240.11268881583865085No Hit
ATTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA676980.111168213083542No Hit
AGGCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA675660.11095145329555671No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA669980.1100187293593776No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT668440.10976584294006145No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT665230.1092387225465518No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC657110.10790532142652112No Hit
GTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA656720.1078412787618891No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT650600.10683630156304827No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT648070.10642084530274314No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG646970.10624021214608874No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG641760.10538466783138926No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC624740.1025897802620639No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA620320.1018639633962344No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA610440.10024154922555668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)