FastQCFastQC Report
Wed 28 Jun 2023
SRR1257249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40851520
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA20139774.929992812996922No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA10788332.640863791604327No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC8307852.0336697386045857No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT6846881.6760404508816318No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT6742061.6503816749046303No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA5649401.382910599164976No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA5554201.3596066927252646No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA4830441.182438254439492No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA4702051.1510098033071963No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA4108851.0058010081387423No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT3903750.9555947979414231No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA3687750.9027203883723298No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG3388460.8294575085578212No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3229250.790484662504602No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA3075220.7527798231253084No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3033430.7425500936072881No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC2583280.6323583553316988No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC2108190.5160615810623448No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2017850.493947348837938No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1920970.4702321969904669No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA1866680.45694260580756846No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1842410.45100157839904115No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1835370.44927826430938184No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1781950.4362016394983589No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1680890.4114632699101527No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1676600.4104131253867665No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC1367800.33482230281761854No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1349600.33036714423355606No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1347820.32993141993247743No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1310360.3207616264951708No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1307050.3199513751263111No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1295890.31721953063190794No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1289530.315662673016818No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1280110.31335676126616585No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1279940.31331514714752356No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1202330.2943170780426285No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1198700.29342849421514794No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1189320.29113237402182346No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1143800.2799895817830034No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1024050.2506761070334715No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA986890.24157975027612194No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA973030.2381869756621051No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG920920.22543102435356138No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC906450.22188892849029854No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT897140.21960994352229735No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG893830.21879969215343764No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT883330.21622940835494003No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT879220.21522332583952813No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT872200.2135049075285326No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA861970.21100071674199639No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA838280.20520166691471944No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA816780.1999387048511292No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA815360.19959110456599902No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT783430.19177499392923444No Hit
TTCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA781850.19138822741479386No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA764810.1872170239932321No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT747160.18289649932242424No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG733990.1796726290723087No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA685050.16769265868197805No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAG651250.15941879274014775No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT650440.15922051370426363No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA647160.15841760600339963No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG626200.15328682996373208No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA614240.15035915432277674No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA612560.1499479089150171No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA605370.14818787648537926No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT600660.1470349206100532No Hit
ATGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC599440.1467362781115611No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA598170.14642539616640948No Hit
TGCTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA597600.14628586647449104No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA592820.14511577537384165No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA591420.14477307086737531No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT587650.1438502165892481No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA581480.14233986887146427No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT574560.14064592945378776No Hit
GTGGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT569730.1394635989064789No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC561810.13752487055561213No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA544350.13325085578211043No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA524570.12840893068360737No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG520270.12735633827088932No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGA518280.1268692082938407No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGAT479770.11744238647668434No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA473440.11589287252959009No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC460310.11267879383680215No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG458250.11217452863443024No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC457050.11188078191460196No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA440890.10792499275424758No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA439270.10752843468247938No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT435590.10662761140833928No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT434780.10642933237245518No Hit
TGTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC434140.10627266745521341No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA433840.10619923077525634No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATA426820.10448081246426083No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG425050.10404753605251409No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC424040.10380029922999194No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA423770.10373420621803058No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC414480.10146011702869319No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG411860.10081877002373472No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA409470.10023372447341004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)