FastQCFastQC Report
Wed 28 Jun 2023
SRR1257250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51091304
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA24708684.836181123895369No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA13350662.6130983072970695No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT9653221.8894056804657011No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC9617211.8823575143041955No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT8775211.7175545176924827No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA7766601.5201412749222452No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6870271.3447043747405625No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA6566851.285316577553002No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA6531011.278301685155658No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6392461.2511835673640272No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG5753051.12603311123161No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT5290271.0354540960630012No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5246821.0269497133993684No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA4219430.8258606983294066No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA4198750.8218130427831711No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG4154750.8132010097060746No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC3866880.7568567832991696No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT3680230.7203241475300768No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC3381990.6619502215093198No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3378960.661357165595147No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2669010.522400054615948No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA2492690.48788928933972797No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2419160.47349740769975257No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2410500.4718024030077604No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2385780.46696400624262785No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2315020.4531142912304607No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG2280560.4463695035068982No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA2213010.43314807545331No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1912220.3742750429701305No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1869920.3659957475346489No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1862010.36444753886101633No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1861300.3643085719636359No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1846270.3613667797557095No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC1777440.3478948198307877No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1773330.34709037765017703No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1682700.3293515467916027No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1638160.3206338205812872No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1604880.31411999192661044No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1495220.2926564567621919No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1446870.28319300677860954No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1407680.27552242549925915No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT1385160.27111463038798145No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG1297450.25394732536088727No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1247850.24423921534670556No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA1219370.23866488120953028No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1200400.23495192058515477No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1190040.2329241782515475No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1187860.23249749115818222No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1181550.23126244732371679No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1173550.229696623127881No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT1155430.22615003132431302No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1070870.20959926957432912No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1064590.20837009758059807No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA1029580.20151765944357186No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1020380.19971696161836072No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT999720.19567322063261489No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA985630.1929154127676992No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG945480.18505693258484848No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA940100.18400391581314893No Hit
TGTCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA904070.17695183509115367No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA879530.1721486693704275No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG867310.16975687291128838No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA863260.16896417441214653No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA825130.16150106483874438No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAG817860.16007812210077865No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC805530.15766479555894677No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA799670.1565178293354971No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA794120.15543153879963603No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA769810.15067339052454015No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA767790.15027801991509163No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA763310.14940115836542361No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA752650.14731469762447247No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT750500.1468938823718416No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA748850.14657093113145048No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC748800.1465611447302265No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC714440.13983592980911194No Hit
GCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACC691440.13533418524608415No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGA673750.13187175649304234No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT667300.13060931073514975No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC658470.12888103227899605No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGAT648970.12702161604644108No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG632590.12381559100546739No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA626090.12254335884635083No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA618450.1210479967393277No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACC612120.11980903834437266No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA603120.11804748612405742No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT590490.11557544117488172No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC583210.1141505411566712No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA580760.11367100749669651No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCA566660.11091124235153599No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA564980.11058241927041049No Hit
CCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA564300.11044932421376444No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT562420.11008135552774304No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT561580.1099169439871803No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG558260.10926712694590845No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG555590.10874453312054826No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA545460.10676180823257124Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT544040.10648387443781039No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA537970.10529580532922002No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG532750.10427410504143719No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA525500.10285507686396103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT517690.10132644099277639No Hit
ATGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC516790.10115028577074485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)