FastQCFastQC Report
Wed 28 Jun 2023
SRR1257263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1257263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37681824
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT12784173.39266220233925No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC9266352.4591033597524365No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA9241682.4525564367584756No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA9090192.412354030420608No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA8768392.326954767370072No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT8610132.284955738872938No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA7127991.89162552216156No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6675051.7714243344483536No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA5873281.5586506640442883No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5584921.4821257060167787No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA5541881.4707037536187209No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA5521271.46523427316045No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT5038731.3371778393742297No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA3629480.9631911661176487No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3289080.872855836277989No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG3129570.8305250828622308No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT2771200.7354208755924342No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2587330.6866254669625335No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA2530510.6715465790615656No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2374760.630213654201028No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2359630.6261984557860044No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAAT2230410.5919060605983404No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2221360.5895043721875035No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA1989020.5278459981130426No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA1925000.510856374680801No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1825040.48432899638828525No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT1546860.41050560609804876No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1493420.39632370237703995No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1442650.3828503630822117No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1411650.3746235851003391No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT1274260.3381630358445493No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1252820.3324732900403123No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA1246410.3307722046576089No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1237680.3284554378259396No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1229850.32637751293567957No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1186760.3149422915408766No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA1183410.31405326875896455No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1169780.31043614024628957No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1142760.30326557440531543No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1136420.30158306561805504No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1118930.2969415705566694No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1089400.2891049010790985No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1068130.2834602698637943No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT974290.25855701677286114No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA930490.24693337562427978No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA894520.23738765936595851No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA893830.2372045472108781No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG871370.23124411387304392No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG870200.23093361934921197No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA841540.2233278304150033No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA840670.22309694987164103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG827240.2195328973459459No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA822360.2182378432636382No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT759600.2015825985493696No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT756830.20084749612969904No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA754100.2001230089074244No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT751500.19943302107668673No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA721530.19147958442776017No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAATA711430.1887992470852791No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC676240.17946052717617916No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAAT636660.1689567893528721No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA635190.16856668084857038No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA631380.16755558329660475No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA630160.16723181977602783No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG621190.16485136175998275No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA614230.16300431741308488No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGAT608220.16140938400434118No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA600790.15943761108804075No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA590750.15677319654165361No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC590010.15657681538982826No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT562350.1492364063905187No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG556360.14764678058047295No Hit
TGCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGA546510.14503278822171664No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA511390.13571264490805965No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC498720.13235028113288783No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC490440.13015293527192315No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA486340.12906487753883675No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG478760.12705329763230147No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT478520.12698960644792565No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA470530.12486922076808173No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATA461600.12249937794943261No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG455050.12076113937584337No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAG447550.1187707898641No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA440000.11676717135561167No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC429730.11404171942419772No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGATC429630.11401518143070782No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGAT420770.11166391520750164No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT417460.11078550762298556No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG416360.11049358969459652No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA408240.10833870462321568No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATC404400.1073196456732031No Hit
GTGGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT396960.10534521895755365No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGA394580.10471361471249374No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAG392490.10415897064855459No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT389810.10344775242302495No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC385490.10230131110426077No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGCTGTAGGCACCAT383940.10188997220516714No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG383100.10166705305985188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)