FastQCFastQC Report
Wed 28 Jun 2023
SRR1258539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28404464
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG310550110.93314417057826No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA14599755.139949129122803No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA7413522.6099841208058No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT6481732.281940613278251No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6042592.127338153608531No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG5437661.9143681077734824No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA5285751.8608870774678234No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA5173241.8212771063027278No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA4856781.7098650409315945No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC4284481.508382626054834No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT3631841.2786159245955144No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2568870.9043895353913385No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG2300350.8098550988323525No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC2298900.8093446156913927No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC1806190.6358824443932475No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1747500.6152201991912257No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1705740.6005182847315831No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1650190.5809614995727432No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1626370.5725754937674585No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1502970.5291316181850853No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1494300.5260792810594841No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1473560.5187776118570658No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1459470.5138171239562908No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1314900.46292019451590427No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1264750.4452645189854665No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1257320.4426487329597207No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA1243490.4377797799669798No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC1212500.4268695230439835No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1109410.3905759320084336No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1029000.3622670014121724No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA948480.33391934450866595No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA940870.3312401881619734No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG923570.32514959620431494No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT900330.31696778365541417No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT769340.2708517928731202No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG733230.25813900237652787No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG674930.23761405953655734No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT626460.22054984033495578No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT589800.2076434183021373No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA573470.20189432196291401No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT554090.19507145074098212No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT536690.18894565304946434No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG526440.18533706532888633No Hit
AAGAGGTGCACAATCGACCGATCCTGTTGTAGGCACCATCAATAGATCGG514140.18100676006419272No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA502420.17688064805588305No Hit
TGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC499290.17577870858608702No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA499140.17572589998529808No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG497540.17516260824354932No Hit
TAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC495890.17458171363487093No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGTCACCATCA493990.17391280469154427No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATATCG477410.16807569401767272No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG474470.16704064544220937No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG467470.16457624407205854No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC466080.1640868843714143No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG465780.1639812671698364No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG455820.16047477607745037No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA455120.16022833594043528No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT449560.15827089713785833No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA438140.15425040233112652No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCATC428850.1509797896555978No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATATCGGA426130.1500221936946249No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG424400.14941313449885904No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCA418050.14717757039879364No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG417600.14701914459642682No Hit
CTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATC413830.1456918884299313No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA412240.14513211726156847No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG407690.14353025637097042No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATTGG407020.14329437795411312No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA402870.14183333999895228No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGAT402310.1416361878893402No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG401550.14136862431200953No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA388320.13671090572242448No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG368300.1296627178037931No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG363650.12802565117933576No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT350540.12341017947038184No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT347400.12230471942719989No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT343560.12095281924700287No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG331960.11686895411932435No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAATCGGA325080.11444679962980465No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC308710.10868362099703764No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGACGGA308450.10859208608900348No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATC306580.1079337388658346No Hit
CCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC302450.10647974205744562No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATCAA297160.10461735873628877No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG287220.1011179087906746No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)