FastQCFastQC Report
Wed 28 Jun 2023
SRR1258540.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258540.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22793695
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG235810510.345426662943416No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA18571418.14760836275119No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA9460594.15052934594413No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6722472.949267330285853No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT5267582.310981172644453No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT4048331.776074480245524No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3349051.469287888602528No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC2998041.3152935493784574No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT2534841.1120794588152558No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2342901.027871961961411No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2231210.9788715695283279No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2102830.9225489768113506No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1909910.837911536501651No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1720520.7548227700686527No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1635010.7173080099562621No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1340440.5880749040469305No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1299690.570197153203989No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1231840.5404301496532264No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1218970.5347838514115416No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1211320.5314276601490018No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA1168370.5125847301194475No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC1099890.482541334347064No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1022420.4485538654439309No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT944400.41432510174414455No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC937760.4114120154718223No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG910830.39959734479205766No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA892180.3914152575964538No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA869770.3815835914273662No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG862200.378262497589794No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG840940.3689353569046177No Hit
TGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC803920.35269402350079704No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC797190.34974145262538614No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCA762940.33471536756107334No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA643100.28213942495940214No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA626140.2746987708662418No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATCAA621810.27279912273986295No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA620580.27225949983098396No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC584880.25659727393913095No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA565560.24812124580942232No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA556880.24431317520042273No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG522650.22929586449235195No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATC486100.21326072846021674No Hit
AAGAGGTGCACAATCGACCGATCCTGTTGTAGGCACCATCAATAGATCGG470330.2063421485634514No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG458840.2013012809024601No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG447620.19637886705073487No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC446700.19597524666360588No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC409420.17961984662864006No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC408110.17904512629479336No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGAT400010.1754915120168099No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT398740.17493434039544709No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA377670.16569055609456915No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG371250.16287398774090817No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGTCACCAT369620.16215887770719054No Hit
CTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATC365700.16043910388377136No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG355520.15597295655662674No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCAT346790.15214295005702233No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG344850.1512918375015547No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGTCACCATCA342850.15041440187736127No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG341030.1496159354593452No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA336690.14771190015484545No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA334960.14695291833991814No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATTGG333610.14636064929358755No Hit
TTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG324460.14234638131290253No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA321640.1411091970827898No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG304030.1333833764117665No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT302480.1327033638030166No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCATC297720.1306150670174362No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT297620.1305711952362265No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAATCGGA297340.1304483542488394No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG297170.130373772220783No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGACGGA296850.13023338252091204No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT296480.13007105693043625No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA293320.1286847086442106No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT289690.1270921629862995No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA289540.127026355314485No Hit
ATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGG287780.12625421196519476No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATTTGTAGGCACCATCAA275990.1210817289605744No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA256760.11264518543395444No Hit
TAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC251260.11023223746742246No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA248920.10920563778711613No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG244690.107349861441947No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG235180.10317765504890718No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT228700.10033476362652041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)