FastQCFastQC Report
Wed 28 Jun 2023
SRR1258541.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258541.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21727393
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG432677819.91393076932884No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG8334973.836157425789647No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7851123.613466189892179No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA4979242.291687732623974No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT3443971.5850820206547558No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT2833511.3041187223888295No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2823411.2994702125561037No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC2502741.1518823266095477No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2426081.1165996767306599No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2146610.9879740289136392No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2013070.9265124444520335No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC1853340.8529969518202207No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1777140.817926016250546No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1709490.7867902053412482No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1658540.7633405443533884No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1387460.6385763814370182No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1367380.6293345915913612No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1270090.5845570151927569No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1124130.5173791443823932No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA1003280.4617581133640838No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT925610.42601061250192324No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC904070.41609685984876327No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA823020.3787937190623836No Hit
AAGAGGTGCACAATCGACCGATCCTGTTGTAGGCACCATCAATAGATCGG735490.3385081680070867No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATATCG717730.3303341546774618No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG690840.31795807255845193No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGACGGA686780.31608946365539575No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT683980.3148007678601846No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT648020.2982502318616872No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC631000.29041680242079665No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA617770.284327714788424Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT617480.28419424272391997No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATTGG617410.28416202532903967No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT601500.2768394717212507No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT580330.2670960110124579No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAATCGGA564520.2598194822544978No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA558320.2569659415651017No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA503770.23185938598339895No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG482180.2219226209053244No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAAATCG472350.21739837816713675No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA448260.20631099184333807No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC415700.19132530073902562No Hit
TGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC407980.18777218233222917No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT404900.18635461695749694No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC395450.18200526864865932No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCA394350.18149899530054064No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC371120.17080742268527108No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA365110.16804132921055No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA360410.16587816126858848No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT355110.1634388442276531No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA354610.16320871997850822No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATCAA353220.16256897456588557No Hit
CCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC345210.15888238409458513No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG344940.1587581170000469No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCAT341950.1573819739901607No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC332640.15309706047108368No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAAAGATCGGA328490.15118702920318144No Hit
CTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATC327160.15057489870045615No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG323590.14893181156156193No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA314220.1446192831325875No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG313340.14421426445409258No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG306150.1409050777513897No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA298140.13721848728008923No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT297390.13687330090637195No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC295930.13620133809886903No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA289710.13333859243950713No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA288890.13296118867090959No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC285920.13159425063098917No Hit
TAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC280790.12923317583476307No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATTAATAGATCGG277250.12760389615081752No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCATCATCAATAGATCGG273770.1260022313767694No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG270530.1245110262423108No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA266960.12286793910341659No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGAT265210.12206250423140964No Hit
GCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC263710.12137213148397509No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG257370.11845415600481844No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG256580.11809055969116958No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA254320.11705039808503487No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCATAGATCGGA242740.1117207204748402No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA236270.1087429126909059No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA227850.10486762033530667No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATATCGGA221240.1018253777616118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)