FastQCFastQC Report
Wed 28 Jun 2023
SRR1258542.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258542.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23351093
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT406745617.418696418193356No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA15874546.798199981474101No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT8999493.8539909031238926No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC5166702.212615914809641No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT4834712.0704426983353628No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4670772.0002361345569564No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4118261.7636262251193124No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3521231.5079508269698554No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA3351631.4353203937819956No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA3069391.3144523898731422No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA2860231.224880565547831No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC2653341.1362808584591737No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2516581.0777140067918876No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG2240460.9594668652126905No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCG1971020.8440804034312226No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1861190.7970462025053816No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG1655140.7088062216188339No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1507740.6456828380581586No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1317760.564324762014352No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAAT1193660.5111794981074333No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG1152520.4935614791136329No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1132260.4848852257151304No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC988210.4231964645081068No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT983430.42114945111991114No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG938680.4019854659479965No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA909980.38969482070924905No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT812970.3481507268203677No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCA812090.34777387079910993No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA799680.3424593444084181No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC749830.32111130729512316No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC651760.27911327319881774No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA610750.26155092611724856No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG581710.2491146774157424No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATCA569000.2436716773814399No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTATGCACCATC524070.2244306080233589No Hit
TAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCATC507840.2174801839040254No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG497050.21285941518883075No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT495930.21237978025268456No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA478950.2051081720243245No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG472300.2022603395909562No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA449130.1923378918494308No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT442440.18947292959691436No Hit
GTTTAGTGGTAAAATCCAACGCTGTAGGCACCATCAATAGATCGGAAGAG398020.17045026543297137No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATATCG367650.15744445024479153No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG358780.15364591284870477No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC348040.14904655640744527No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAA334810.14338086872421774No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATAGA334050.14305540216040422No Hit
TTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGG331310.14188200954876073No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG305830.1309703147514337No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT291400.1247907324937638No Hit
AATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATC288080.12336895750447313No Hit
TTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAGA284380.12178444923327574No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC281570.12058107943812307No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA279740.11979739021209843Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGCGTCGCTGAACCATAGCAGGCTAGCACTGTAGGCACCATCAATAG272280.11660267894098147No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT268110.11481689529479412No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA267330.11448286382140657No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG258680.11077854043063423No Hit
TGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAGA252630.10818765528448711No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA249490.10684296448136281No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG243730.10437627052403928No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA240260.10289025871294333No Hit
TCTAAGTCAGAATCCATGCTAGAACGCGGTGATTCTGTAGGCACCATCAA236390.10123294871036657No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)