FastQCFastQC Report
Wed 28 Jun 2023
SRR1258543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7341479
Total Bases374.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1886272.5693324192577545No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1434451.9538978453796572No Hit
ATGTACACGGATTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG249120.33933216998918064No Hit
ATGTACACGGATTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA190100.2589396496264581No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA134380.1830421363324747Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC119860.16326410523002244No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAG77590.10568715104953648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)