FastQCFastQC Report
Wed 28 Jun 2023
SRR1258544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1258544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7912713
Total Bases403.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1192521.5070937110950442No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1005481.270714608251304No Hit
ATGTACACGGATTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG155460.19646864482510615No Hit
ATGTACACGGATTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA134620.17013128114213166No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA84100.10628465862467147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)