FastQCFastQC Report
Sat 2 Sept 2023
SRR12651177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12651177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105934120
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG79164147.472959609236382No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA58655255.5369554209729595No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG31988243.019635222343849No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA21324182.0129661718056466No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG17159681.6198444844777111No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC15155041.4306098922613413No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA13509561.2752793906250413No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11872951.1207862018394072No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG11241061.0611368650629278No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG10641781.00456585659087No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA10260280.9685529081659431No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA9783420.9235381386091658No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA8610960.8128599170880921No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA8608930.8126682885551888No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8287720.7823466131591974No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT7951090.7505693160994776No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA7077780.6681303436513184No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG6179170.5833030944137734No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA4812340.4542766768629409No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA4772750.4505394484798666No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT4600370.43426707089273975No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA4554470.4299341892867001No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA4434090.41857052288724356No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA4292380.4051933409179215No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA4099100.3869480390265195No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA3330110.31435669640716324No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG3278620.30949612834844903No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3219470.3039124693724742No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3077300.29049186418879963No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG2866260.2705700486302241No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG2819120.26612011314201695No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2775390.2619920758297704No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2685350.2534924536117353No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG2654180.2505500588478953No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2619730.24729803768606373No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA2606050.2460066690505382No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG2460010.2322207424765505No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2344230.22129130822061863No Hit
GTGGTGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2319450.21895211854310961No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2261390.2134713537054917No Hit
CCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC2181070.20588928288638259No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA2158330.20374266572469757No Hit
GCGGTCGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2083120.19664297017806917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2071190.19551679855366713No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC2043180.19287270239277016No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2007990.1895508264948064No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1972660.18621573483595275Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA1945950.18369435645474752No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1869490.1764766630430309No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1866340.17617930842300855No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1833040.1730358452970582No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1822870.1720758146667004No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1721610.16251704361163333No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC1715830.16197142148346538No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1712100.16161931585404213No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC1648090.15557688117860421No Hit
CCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1624940.15339156071717025No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1618960.15282705893058818No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGA1597650.15081543132656408No Hit
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1562490.14749638737736245No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1549450.14626543364876207No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1537760.145161917614457No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1413700.13345086550018068No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG1412530.1333404194984581No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1390030.13121645792687003No Hit
AGTCCGCCCGGAGGATTCCACCCGGCGGCGGGCTGTAGGCACCATCAATA1362480.1286157849803255No Hit
TTGGTGACTCTAGATAACCTCCTGTAGGCACCATCAATAGATCGGAAGAG1360970.12847324355929893No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1346330.12709125256338563No Hit
TTGGTGACTCTAGATAACCTCGCTGTAGGCACCATCAATAGATCGGAAGA1338880.1263879852874598No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1338800.12638043342409414No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA1317970.12441411700026395No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1311130.12376843268250116No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG1294370.1221863173073982No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA1268050.11970175426010052No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1267070.11960924393387136No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1263060.11923070678266831No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1255750.11854065526763237No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1250170.11801391279787853No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1229040.11601927688642714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1212690.11447586481107314No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA1202380.11350261841982544No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1192160.11253786787486411No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA1188280.11217160250163026No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC1176060.11101805537252775No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1163560.1098380767216455No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1145410.10812474772056443No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA1131090.10677296417811373No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGAT1130880.10675314053677891No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1101880.10401559006673203No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG1096400.10349828742618526No Hit
AAATGGATTTTTGGAGCAGGGCTGTAGGCACCATCAATAGATCGGAAGAG1094380.10330760287620268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)