FastQCFastQC Report
Sat 2 Sept 2023
SRR12651178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12651178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107074416
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG44425894.149066757459597No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA37474433.499849114283285No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA33595793.137611322577748No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG22379602.0900977876918794No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA19448601.8163629302447002No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC16659131.5558459828536444No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA15088451.4091554793070271No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT13692731.2788050135150866No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA13486751.2595679251708456No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12235851.1427426323763465No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC11165741.0428018584756977No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG10381850.9695920265397479No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA10242610.9565879864336593No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA9801030.9153475093434085No Hit
CGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG9717660.9075613356602384No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC8830320.8246899987761782No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA8643470.8072395183551597No Hit
CCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGC7198050.6722474208965099No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT6819120.6368580147100685No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAA6081040.5679265157047413No Hit
TGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAG6079010.567736927932439No Hit
CCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA5131750.4792694830107688No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC5022350.46905228976453167No Hit
TCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA5015040.4683695869982611No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4957690.46301349894824545No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA4852550.453194159844869No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA4749630.44358215318213834No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4703610.43928420772334636No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG4663940.43557930775919435No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4510000.4212023906812623Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA4327010.40411240720659175No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGAAG4236060.3956183146495051No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG4230300.39508037101972143No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC3851810.3597320577494441No Hit
CTGTAGGCACCATCAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3832750.3579519873356115No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGAA3709730.3464627815481151No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3494720.3263823544925989No Hit
TCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3322540.3103019492536854No Hit
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3043200.28421355106900603No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG2886780.269605019372695No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC2782740.25988841255972855No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC2722840.25429417238194413No Hit
CGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA2714530.2535180766243918No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT2668650.2492332061843793No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2495240.2330379275661891No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2420440.22605213181830477No Hit
CCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2362600.22065028120256103No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2342060.2187319891616313No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2306910.21544922551807333No Hit
TTTTGGTGACTCTAGATAACCTCCTGTAGGCACCATCAATAGATCGGAAG2181770.20376202658905934No Hit
GCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA2091820.19536132702325454No Hit
TTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAGATCGGAAGAG2090850.19527073582171112No Hit
TTTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAGATCGGAAGA2079390.19420045214162085No Hit
CCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA2046580.19113622809766248No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1950420.18215555805599723No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1923920.17968064378702753No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1884480.17599722421087033No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1878640.17545180914178415No Hit
TGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGAA1868520.17450667207001155No Hit
TTTGGTGACTCTAGATAACCTCCTGTAGGCACCATCAATAGATCGGAAGA1860580.17376513171923347No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1857500.17347748130608528No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG1811250.16915805545929852No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1785160.16672143231675435No Hit
TTTGGTGACTCTAGATAACCTCGCTGTAGGCACCATCAATAGATCGGAAG1768980.1652103337178136No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA1744100.16288671609472052No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA1724740.1610786277835034No Hit
GGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAAGA1718330.16047997870938657No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCG1669590.15592800431430792No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG1649310.15403399445111146No Hit
TTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAGATCGGAAGAGC1630200.1522492543877148No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1621510.1514376692934753No Hit
GCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1592230.14870312250874196No Hit
ACCATGATCACGAAGGTGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1568480.1464850389657974No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1567810.14642246566163855No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1548940.14466013991614954No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1513920.14138951736145822No Hit
TTGGTGACTCTAGATAACCTCCTGTAGGCACCATCAATAGATCGGAAGAG1509490.1409757864100795No Hit
TTCTGATCGAGGCCCTGTCGGCACCATCAATAGATCGGAAGAGCACACGT1486530.13883148333024764No Hit
CTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1464300.13675535713405151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1440650.13454661289023515No Hit
TTGGTGACTCTAGATAACCTCGCTGTAGGCACCATCAATAGATCGGAAGA1344310.12554913211013918No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1330040.1242164141245468No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA1326020.12384097429959368No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1325300.12377373134587072No Hit
GTGGTGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1284670.11997917411008807No Hit
GTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1236920.11551965877637847No Hit
TTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1227330.11462401998998527No Hit
TCGGCCGAGGTGGGATCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC1206100.11264128678507104No Hit
TGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACA1202640.11231814703523575No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1195210.1116242371100114No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1182560.11044281577029567No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG1164400.10874679904861681No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1162110.10853292909858131No Hit
GTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1160370.10837042529375084No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC1149070.10731508449226565No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG1148600.10727118978636316No Hit
GGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAGA1135010.10600197903484247No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1119760.10457773591779383No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG1101510.10287331382689961No Hit
CGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGAAG1087010.10151911545331238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)