FastQCFastQC Report
Sat 2 Sept 2023
SRR12660872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12660872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66549287
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length1-68
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG35504505.335068428306377No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC25733593.8668468378932443No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC14298652.148580494934529No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG5833390.8765518404427082No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5441770.8177052295090704No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3483630.5234661642580783No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2580560.38776673895845043No Hit
CACATATACTAAAATTGGAACGATACAGAGAAGA2409110.36200387841871245No Hit
CACATATACTAAAATTGGAACGATACAGAGAAG2215760.3329502237942835No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1871070.28115552913436925No Hit
CACATATACTAAAATTGGAACGATACAGAGAAGAT1766020.2653702360477581No Hit
CACATATACTAAAATTGGAACGATACAGAGAA1404200.21100150930242123No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1188510.17859094418246735No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC1058300.15902499451271357No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1055660.15862829604770973No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGAT1022640.1536665599437602No Hit
ACATATACTAAAATTGGAACGATACAGAGAAG1020360.15332395672398413No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGA1019620.15321276094212702No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCT963080.14471680214996144No Hit
GCACATATACTAAAATTGGAACGATACAGAGAAG920630.13833807115018376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG806270.1211538149161538No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA793530.1192394442933701No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG758020.11390354940992832No Hit
GCACATATACTAAAATTGGAACGATACAGAGAA715020.1074421728966082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)