FastQCFastQC Report
Sat 2 Sept 2023
SRR12660873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12660873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64435036
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length1-68
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG17083292.6512424079347143No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC14187772.2018719753644587No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5207180.8081286708678179No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG3295810.51149346762218No Hit
CACATATACTAAAATTGGAACGATACAGAGAAG2094560.32506538833935006No Hit
CACATATACTAAAATTGGAACGATACAGAGAA1633850.2535654670853292No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1386810.21522607669529353No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA1343480.20850147426005938No Hit
ACATATACTAAAATTGGAACGATACAGAGAAG1195930.18560244150402896No Hit
CACATATACTAAAATTGGAACGATACAGAGAAGA1181190.18331486615449397No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC903010.14014270124719105No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGA883910.13717847538721015No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTG871060.1351842187222492No Hit
ACATATACTAAAATTGGAACGATACAGAGAA846020.13129813413932134No Hit
GACTGCATAATTTGTGGTAGTGGGGG763520.11849454076505832No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720250.11177925003409636No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC657140.10198488909046313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)