FastQCFastQC Report
Mon 10 Jul 2023
SRR12671761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35004024
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG34813729.945633679145004No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT26168197.475766214764337No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG18768155.361712127725658No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT12925613.6926068842827897No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA12909773.6880816902650966Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG8614152.460902780777433No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT7723612.206492030744808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA7675782.192827887445169No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC5781641.6517072437157512No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5013121.4321553430542728No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC4656861.3303784730578405No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT4226811.2075211695661048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3638791.0395347689168537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG3632391.0377064076975835No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA3288470.9394548466770564No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC3025450.8643149141938653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT2901040.8287732861798975No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2596940.7418975601205164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2504770.7155663017486218No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2405440.6871895642626688Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2372650.6778220698283146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2234130.6382494766887373No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2225880.6358926048045219No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC2186710.6247024627797079No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2101390.6003281222753133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1981720.5661406242893674Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1862640.532121678353323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1747860.49933116261147575No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1739460.4969314385111837No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1642580.4692546205544826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1592110.45483627825189465No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1567000.44766281728066465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC1508880.431059011958168No Hit
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1467470.41922894350660944No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1151220.3288821879450203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1065670.3044421407093082No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1058960.30252521824347967No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1039490.29696300059673136No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1009800.28848111862796116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA967980.2765339207857931Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC965200.2757397263811726No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA940330.2686348289556652Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA901530.2575503890638402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA883340.252353843660946No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA873550.24955702235834373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCACAC843620.24100657684385088No Hit
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT835130.23858114141391287Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA833850.23821546917005884No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA812600.2321447385592011No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCAC773500.22097459423522278No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT765300.21863200642303296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG764080.2182834750656096No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG744930.21281267547982483No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT738120.2108671848699452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC731870.20908167586675178No Hit
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT719380.20551351467476994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA705620.20158253805333923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG678170.19374058251131354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG640080.18285897644225133No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA638900.1825218723424484Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC610370.17437138084467088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA606820.173357211730857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC594640.1698776117854336No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA584600.16700937012270362No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT581460.16611233039949921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG571760.16334122042654295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG568650.1624527511465539No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG567880.16223277643736045No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC552330.15779043003741514No Hit
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT533510.152413905326999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG530310.15149972471736392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA521150.1488828827222836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA518810.14821438815148796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA501570.1432892401170791No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA495900.141669426349382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA473650.1353130142980133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA470290.13435312465789648No Hit
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT457110.1305878432719621Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG457070.13057641601434167No Hit
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA452870.12937655396419567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT446170.12746248831277227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG427840.12222594750820649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC427160.12203168412865904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA411410.11753220144061151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC403300.11521532495806769Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG386530.11042444720069898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC385850.11023018382115153No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC376550.10757334642439967No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA375380.10723909913900186No Hit
ACCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT375170.10717910603649455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA366840.1047993796370383No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC366420.10467939343202369No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA360830.10308243417956747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCT353100.1008741166444178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA352350.10065985556403459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)