FastQCFastQC Report
Mon 10 Jul 2023
SRR12671762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36100849
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG466349812.917973203344884No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT24946256.910156046468602No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG23218306.431510793555022No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT14096733.904819523773527No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG10917893.0242751354684207No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA8124332.2504539990181396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA6928741.9192734220738132No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG6591141.8257576158388962No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT5111961.4160220996464654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3366200.9324434447511193No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC3365190.9321636729374425No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3032380.8399747053040221Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC2974590.8239667715293898No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2926410.8106208250116222Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA2908410.8056347926886707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2875250.7964494131426105No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2795180.7742698793593469No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2743950.7600790773646348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2406550.6666186714888617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2332410.6460817583542149No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2302340.6377523143569283No Hit
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1982980.5492890208759357No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1870270.5180681484803862Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1711280.4740276329789363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1701450.4713047053270132No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1699000.4706260509275004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1650200.4571083632963867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1569660.4347986386691349Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1535550.42535010741714135TruSeq Adapter, Index 6 (100% over 35bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1497030.41467999824602464No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1487270.4119764607198019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1451960.40219552731294494No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1408590.3901819594325884No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1405550.389339873973601Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1360290.3768027726993346No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1344930.37254802511708246No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCTG1304770.3614236330009857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1078320.29869657636029556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG1037060.2872674822689073No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC991230.27457248997108075No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA988410.2737913449071516No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC963430.26687184005007747No Hit
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG900110.24933208634511617No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC894800.24786120680984539No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT843970.2337812055334211Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG835500.23143500032367662No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA816880.22627722688737875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA811840.22488113783695227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG809360.22419417338356779No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA807850.2237759006720313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT805410.2231000162904756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA756680.2096017187850624No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA732450.20288996527477787Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT732450.20288996527477787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG724230.20061301051396327No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA722940.20025567819748508No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA720500.19957979381592938No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT705320.19537490655690673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA696500.19293175071866037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA684570.18962711929572626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA678110.187837687695378No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA633980.17561359845027466Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCT630540.17466071227299945Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG621260.1720901356087221No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC619950.1717272632563295No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG607350.1682370406302633Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA598680.16583543506137485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT584150.16181059896956995Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA547490.15165571313849158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG536450.14859761331374782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA531050.14710180361686231Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG527670.14616553754733025No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG523640.1450492203105805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA506260.14023492910097488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACAC505090.13991083699998302No Hit
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC502630.13922941258251295Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA501530.13892471060722145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG488680.13536523753222535No Hit
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT483950.13405501903847192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC480900.13321016356152732No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT475010.1315786229847392Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA471140.13050662603530458No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC457490.12672555152373285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA447570.12397769371019503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC441670.1223433831154497No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC433670.12012736874969339No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT426900.1182520665926721Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTG416600.11539894809676082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT416310.11531861757600216TruSeq Adapter, Index 8 (97% over 36bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA410740.11377571757384432No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGAGATCGGAAGAGCACACGTC407870.11298072242012923No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC403310.11171759423164813Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTC399230.1105874269051124No Hit
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA396080.10971487124859586Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC392550.10873705490970587No Hit
CCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA391540.10845728309602913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGT383420.10620802851478645No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG374320.10368731217373862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACA369220.10227460301556897No Hit
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT365740.10131063676646497Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)