FastQCFastQC Report
Mon 10 Jul 2023
SRR12671763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48102432
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC22004484.5745046736930055No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC8937311.8579746653973754Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT7735811.6081951947876565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA5418661.1264835840316763No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT5085681.0572604728176738No Hit
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA3305760.6872334438308649TruSeq Adapter, Index 10 (100% over 35bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT3274440.6807223385295779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3246830.674982504003124No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3080480.6404000529536635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2870250.5966954020121062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC2599030.5403115584675635No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC2557510.531679978259727No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2425550.5042468538804857No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG2391110.4970871327254306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2376710.4940935210926549No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG2170250.451172614307734No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2153910.4477756966633205Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2141060.44510431406046164Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC2012540.4183863302379389No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2004000.41661095222794553No Hit
CCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTC1869110.38856871103731305Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1759340.3657486590283003No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG1742210.36218750852347753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1718410.3572397337415289No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1648120.3426271669590427No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA1597760.33215784183219677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1530050.3180816304672496No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1458610.3032299905335348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA1451070.301662502220262Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1375440.2859398044572881Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC1291290.2684458864782554No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1273000.2646435839252369No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1252380.2603568983788595No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1251090.26008872067009003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1239130.2576023598973125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC1220920.2538166885200316No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1201100.24969631473103063Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG1179830.24527450088178493No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG1146550.23835593177492564No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1093220.22726917424881968No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGAGATCGGAAGAGC1080530.22463105399743613No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1079050.22432337724628973No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCA1066010.221612495601054No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG1053940.21910326696163723No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1051120.2185170180168853No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1048120.2178933489267237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1035170.2152011773541928Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG994660.20677956574004408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG984740.2047172999485764No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT976400.2029834998779272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA940990.19562212571705315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG936440.19467622759697473No Hit
TCCCCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAA935590.19449952135476226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTC934550.1942833160701729No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG873360.18156254552784357No Hit
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC872090.1812985256130085No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG855810.17791408135039824No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT845420.17575410740147193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGT822450.17097888106780132No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT816190.16967749156633077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT806780.16772124952019057Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC796130.1655072242501169Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT786240.16345119514955087No Hit
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC764990.15903353909423956No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG761140.15823316376186553No Hit
TGATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT757510.15747852416276997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA751440.15621663370367636No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA749210.15575303967998957Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACA743340.15453272716024005No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA737790.1533789393434411No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC732930.1523685954173793No Hit
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA732920.1523665165204121Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA729410.15163682368492304No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTA720420.14976789531140547TruSeq Adapter, Index 3 (97% over 39bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG700380.145601785789126No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT700320.14558931240732279Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA697630.14503008912314455TruSeq Adapter, Index 3 (97% over 36bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG696390.14477230589921108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA692120.14388461689421442Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA678250.14100118680070065Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGA670680.1394274617965262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT666450.13854808837939836No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA660350.13727996122940314No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC642970.13366683830040027No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA641210.13330095243417214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG634720.1319517483024559No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCAC620940.12908702828164698No Hit
CCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACT620150.12892279542123775Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTC617180.1283053630219778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACTCC578410.12024547948012276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA567290.11793374605259044No Hit
CCCCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAAC556470.11568437953407427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTC540640.11239348563498827No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA538980.11204838873843219No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT536480.11152866449663085No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG530270.11023766947999636No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT521940.10850594830631433No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG521460.10840616125188847No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC519600.10801948641598828No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAG518790.10785109576164464No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA518040.10769517848910426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG513240.1066973079448457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC508190.10564746497640702No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA507420.10548738990993221No Hit
GCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC501350.10422549945083859No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT500050.10395524284510188No Hit
AGGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCC496350.10318605096723592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA494620.1028264017919094Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC490230.10191376602330628No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC486900.10122149333322689No Hit
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCACAC482170.10023817506773877No Hit
TGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC481810.1001633347769194Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)