FastQCFastQC Report
Mon 10 Jul 2023
SRR12671764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35993109
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG371828710.330552439912873No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT28616147.9504496263437545No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG19416305.394449254161401No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT12339393.428264560307919No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG9091282.52583904324575No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA8543612.3736793617911696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA6453421.7929598690682707No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT6306771.752215958893687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC5055571.4045938626752137No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC4152911.153806968995093No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3504870.9737613941601988No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC3317350.9216625326809084Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3144520.8736450079930578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG2967590.8244883763722661No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2871970.7979221800484086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA2779310.7721783633639427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2753740.7650742257358207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2673420.7427588430885479Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2431950.6756710013575098No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2289590.6361189859981253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC2117720.5883681790311585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2072190.5757185354563286No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1959270.5443458635373788Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1931940.5367527434209698No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1762620.48971040540010036No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1724150.479022248397603Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1707790.47447693390420925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1640670.4558289199190878No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1618890.44977776162653804No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1575060.43760043068243976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1511090.41982758421896815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1456650.4047024667971861Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC1372590.38134799636230365No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1359730.37777509022629857No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1344890.3736520787909708Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1320640.36691467802906386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1222920.33976503669077324No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1078180.29955178364836443No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA1062440.29517872434970815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1010650.28078985896994896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA987490.27435529395362873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT937480.26046096768134147No Hit
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT935540.2599219756203889Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG914850.25417365307342576No Hit
CTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTGA895350.24875594936797485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA888140.24675278815175428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC887540.24658608957620196No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC824320.22902161633217072Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT823440.22877712508802728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA778430.21627195361200946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA769650.21383259778976027No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC758580.21075700907081962No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA755050.20977626578465336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT752340.20902334388507535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG747970.2078092225931358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT737120.20479475668523103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA731120.20312777092970768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCACAC729050.20255266084405213No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG716780.19914367497400684No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA682710.18967797419222665No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA668080.18561330725834216No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC663260.18427416203473837No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA662720.18412413331674127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG660960.1836351508284544No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA648730.1802372781967793Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG632660.1757725346815692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC626110.17395274189845616No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA611450.16987974003579406No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT609670.16938520092832213Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG608350.169018464062107No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG606780.1685822694560784No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG597860.16610401729953364No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA595490.1654455579261019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT594310.16511771739418232Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA581130.16145590535121598No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC570670.15854979351742024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC569490.15822195298550062No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC567320.1576190598039197No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC562740.15634659401053685No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA542050.15059827146357377No Hit
CTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGAAC528680.1468836715383492Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA524240.1456501020792619Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA520620.1446443540067628No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG467640.12992486978549142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCTG466130.12950534503701805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA463780.1288524422827714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTGAACT460300.12788559054456786Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG458980.12751885367835272No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA458750.12745495255772432Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG453680.12604634959430708No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG447450.1243154627181553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA442320.12289018989718281Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT439870.12220950404701078Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT435600.12102316585099664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA424760.11801147825268442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACAC414750.11523039035055294No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA383800.10663152216164488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA366350.10178337192266443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA366350.10178337192266443No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCAC361580.10045811824702335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)