FastQCFastQC Report
Mon 17 Jul 2023
SRR12671766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35724001
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG402972711.280167078709912No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT22612556.329792119309369No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG19699145.514259167107291No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT13677253.8285885167229727No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA10248352.8687576176027987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG9409262.633876311894628No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA6082661.7026816229234794No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT6036241.6896875576730612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5071741.4197010015759433No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT5014121.4035717891733348No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA4728331.3235723512604314No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC4414321.2356734622194194No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG3883501.0870842826367628No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3189930.8929374959988384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC3070810.8595929666444697No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2769860.7753498831219942Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC2703230.7566985568049894No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2674170.7485639696404667No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2636670.738066825157686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2372710.6641781249530253No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2301360.6442055580504547No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2300190.6438780471425919Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2241340.6274045284009482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1986500.5560687337344996No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1966800.5505542338328789Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1966130.5503666848514532Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1837590.5143852728030099Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1820950.509727339891184No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1784500.49952411545392134Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1736180.4859981948830423No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1728290.48378959568386537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1703420.4768278894628852No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1632590.45700088296380914Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1611690.4511504744387394No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1297580.36322359301244No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC1184540.331581000683546No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1135200.31776955778273547Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1128000.31575410604204157No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1121270.31387021851219854No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1109620.3106091056262147Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1078860.3019986479118058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1040310.29120758338350733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA991470.27753610240913384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT931620.26078265981461596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT821260.2298902634114247Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA805850.22557663683863408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA803570.22493841045408103Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG787540.22045123109250836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG755820.2115720464793403Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT726300.2033086943424954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC722470.20223658598598743No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC722430.20222538903187245No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG707280.19798454266082904No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA703470.19691803278137854No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA702330.196598919589102No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA681110.19065893543111256Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT680120.19038181081676714Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG679990.1903454207158935No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA671200.18788489004912973No Hit
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG669590.18743421264600235No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA643650.18017298790244687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG640250.17922124680267476No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA603710.1689928292186533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA590670.16534262217717438Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG545080.15258089372464187No Hit
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT536770.15025472650725769Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC535900.15001119275525718Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG519010.14528327888021278No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT511290.14312226673602432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC507240.14198857513188404No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA487090.13634810949646992No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA456900.1278972083781993No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC447230.12519034472090626No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG444710.1244849366116634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT444090.12431138382288144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG443970.12427779296053654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGT429950.12035326054324096Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA427060.11954428060843465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA424090.11871290676539843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC423670.11859533874719129Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA418100.11703616288668225No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT415700.11636434563978429Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT414800.11611241417219756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA412740.11553577103527682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA407880.11417534111030844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC404820.11331877412051354No Hit
GTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA399080.11171201120501592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA390330.1092626774923671No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCAC383720.10741238082486897No Hit
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA379380.10619751130339515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG377720.10573283770762407No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC370260.10364460576518292No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC359850.10073059845676301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)