FastQCFastQC Report
Fri 7 Jul 2023
SRR12671769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38575732
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG404309010.48091582552471No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG20713755.36963238960702No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT19130114.959104858982326No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT10845912.8115889025774026No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG10051832.6057392767038094No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC9850772.553618425179851Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA7344931.9040286779263191Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA5738771.4876632801161103No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT5461751.4158512922061983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG3975101.0304665119510887No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA3782730.9805983720542231No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT3476660.9012557428592671Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC3303020.8562429871713129No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA3004440.7788419931992476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2928870.7592519566446594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2727270.7069911207388105No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2644010.6854076028939645No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC2508430.650261153825934No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2464340.6388316882748978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT2452250.6356975935025679No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2341610.6070163490351913No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2202020.5708303863164541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2195640.5691764967674495Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1945910.5044389047497531No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1930010.5003171423940834No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1926400.4993813208781106No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1858970.48190141926535573No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1841650.477411549831381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1770100.45886361923086777No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG1695360.4394887438558522No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA1570330.4070771748414262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1522070.39456671878579No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1478440.38325649918969784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1407800.364944468195704Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1393690.3612867281429682No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1359290.3523692045558591Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1336850.3465520757972914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT1249790.323983482672474Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1176900.3050881834206024No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1172110.3038464701071648No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1171540.3036987088151691Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1119360.29017206983914134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC1050580.2723422072716598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1036150.268601513511137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCAGATCGGAAGAGCACACGTCTGAACTC1018150.26393536744811474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG981920.2545434523445984No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA956570.24797196330584212No Hit
TCCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT921490.2388781630896855Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCTG920780.238694109550533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC889140.23049206169308725Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTCCCCGCTCGGCGGGGTCCCAGATCGGAAGAGCACACGTCTGAACT873540.22644806843846801Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC847860.21979103338855632No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA845550.21919221131046848No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA838290.2173101990650495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT820940.21281255272096977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA800610.207542399973123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA747340.1937331999299456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC739290.19164639571842731No Hit
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG716170.18565299033081212No Hit
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG678400.17586186050857053No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGT674420.17483012376796894No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG670790.17388911764525944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC664820.1723415125343571No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA654700.16971810152559128No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA653770.16947701731233514No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA650310.16858008034688754No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTG645390.16730466708966144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA640200.16595926164149002No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTC632770.1640331802388092No Hit
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT629070.1630740279925213Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA625700.16220042175738883No Hit
TTGGGGAAGGTGGCTCGGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC625260.1620863604091816Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG625130.1620526604653931No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC620490.1608498317024807No Hit
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT609180.15791793659288175TruSeq Adapter, Index 1 (100% over 35bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG605600.156989891987014No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA604390.1566762232794442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCAGATCGGAAGAGCACACGTCTGAAC590790.15315069069849407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA580800.15056097963351675Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA572890.14851046766915532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG571060.14803607615274805No Hit
CTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC548080.1420789630122897Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA542240.14056505784517584Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT534640.13859490728523313Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT515590.1336565693685346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA513990.1332418008295993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA501030.1298821756642233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC498100.12912263077729802No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGAGATCGGAAGAGCACACGTCTGAA494700.1282412476320605Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT482580.12509937594962553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG471590.12225043454781365Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACAC465040.12055247584154723No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA453470.11755318084437126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC443540.11497902359960402No Hit
TTCCGTCCCCGCTCGGCGGGGTCCAGATCGGAAGAGCACACGTCTGAACT439680.1139783944994226Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC430920.11170753674875178Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC427930.11093243804161641Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA426730.11062136163741494No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC426230.11049174646899766No Hit
CTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTGA418110.10838679613390098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT413910.10729802871919578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA409380.1061237152933352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT394580.1022871063081836Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC392340.10170643035367417No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG386000.100062910018143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)