FastQCFastQC Report
Mon 10 Jul 2023
SRR12671770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41525992
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG35528988.555841363163582No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT27066606.517989985645617No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG19526004.70211524386943No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA15786913.801693647679747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT12012292.8927159644976093No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT9942592.394305234177187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA9344852.2503616530099992No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG9016942.171396652005327No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC8009151.928707687464757No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6172841.4865003104561596No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC5353801.289264805522286No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT5331631.2839259806243761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC5034641.2124069185391164Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT3864970.9307351405355953No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA3835640.9236720943355188No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3737490.9000362953400367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3206530.7721742083849557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA3156290.7600757617060659Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2793390.6726847127456943Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2768520.6666956926640066No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2747700.6616819653579858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG2716440.6541541500080239No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2377290.5724824105345876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA2188580.5270385834491323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2167300.5219140821488383No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2163790.521068828409927No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2031390.4891851831017065No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT1970620.47455097520608297No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC1894020.45610469702927264No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1881700.4531378804869972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1873250.4511030103748034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1737260.41835484628518926Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1733740.4175071844159677No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1721650.41459575487082884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA1705970.4108198065442964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1653800.398256590715521No Hit
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1617790.3895849134681719No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC1556020.37470989254151954No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1491000.35905222926402336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA1325160.31911579619819797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1275870.3072461218987857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1229530.29608684604090857No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1219890.2937654084217904No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1158140.27889520375575855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1157550.2787531240674515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT1103380.26570828217661846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG1089330.26232485909066305No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1041120.2507152628647619No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1020460.24574006564370576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1017970.2451404411964439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1013200.24399176303843625No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC1000930.2410369871477122Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA950750.22895298925068425No Hit
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT949110.22855805587979694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCACAC911800.2195733216921103No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT904890.21790930364770095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG882300.2124693372767591No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA873540.21035981512494634No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC859270.20692341317216453No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC820400.19756301065607293No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCAC802650.19328857935531077No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG784630.1889491285361708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG763830.18394021749077058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA748460.1802389212038571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA739120.1779897274940476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA735040.17700721032744984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG722350.1739512929636937No Hit
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT707390.170348730019502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA697130.16787798832114595Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT673750.16224777965569132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG667050.16063433234779798No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA656750.15815395812820077Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC642950.15483073830000257No Hit
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT616270.1484058466321527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA611160.14717529204359525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG605540.14582192280921308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA590650.14223621677719342No Hit
GTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA583000.1403939970898227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG564160.13585707958523904No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA561700.13526467952890806No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA557560.1342677135804486No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG556310.13396669729166252No Hit
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA554720.13358380457232666TruSeq Adapter, Index 7 (100% over 35bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA546390.13157783202385628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT499340.12024757891394863Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC498350.12000917401323008Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA490460.1181091591984124No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA488290.1175865949210798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG486570.11717239650771015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT482770.1162573069898005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA481520.11595629070101443Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC470850.11338681565993655Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG469340.11302318798308297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA451190.10865243146990924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC449890.10833937452957175No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG446920.10762415982741604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG432910.10425036926270177No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT429180.10335213665696415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA418210.10071041770657761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG415990.10017581277769355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)