FastQCFastQC Report
Fri 7 Jul 2023
SRR12671771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49771387
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG544666210.94335988667545No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT30860986.2005465107894215No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG25867635.197289358241112No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT19528353.923609763979453No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG12374702.4863080468302No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA9047641.8178396354515898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA7201891.4469940329370368No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA6497501.3054689434312932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT5990671.2036373428773444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC5619581.1290784401889382No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC5394631.0838817893501742No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5176501.0400554037202139Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG5113291.0273553357072407No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA4950720.9946919904000264No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA4040570.8118258789934867Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA3813750.7662535102748894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT3485180.7002376686830126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3248570.6526983063582295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA3244120.6518042183554177No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3078310.6184898966146956No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT2921130.5869095028434711No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2787340.5600285963499471No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2731730.5488555100945851Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC2718630.5462234757492291No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2689140.5402983846923937No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2420440.48631154281475014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2362260.4746220956229329No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2304210.4629587678559169No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2280150.45812466508116406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT2238960.44984882579221674No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2233400.4487317180853328Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2028360.4075353576142051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC2013680.404585871798188Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1877880.37730111881350625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1842790.37025088330369416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA1548750.3111727627763317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1548700.3111627168437158No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1469640.2952780881915145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA1426390.2865883564787937No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1394410.2801629779776883Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1305920.26238368643413534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1276400.2564525678177303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT1190770.2392479036198047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1187510.2385929088132505TruSeq Adapter, Index 5 (100% over 35bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1182410.23756822368643252Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCTG1166350.23434147013021758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG1150770.23121115752711494No Hit
TCGGGGTCGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1148100.23067470472542786Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1119070.22484203624865828No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1086170.21823181258742094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1055090.21198726087340103No Hit
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT993440.1996006259580429Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG931220.187099467410864No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG921360.18511840949901598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCACGGCCCTGGCGGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC920080.18486123362404988Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC914110.18366174926971596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC897170.18025818729946183No Hit
CCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC882250.17726048100688854Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT858510.1724906722008772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC856080.17200243987574626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC829200.16660174650145876No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA822710.16529778444791984No Hit
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG819520.16465685394702784No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACG817460.16424296152325432No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA813090.16336494701262796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA808750.16249296006157113No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC806400.16202080122862558No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG795690.15986896246230792No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA789150.15855495447615314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA773060.15532217336036866No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA749480.15058451153872807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA731350.14694185637221643Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA730190.14670879073552842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG726920.14605178674245103No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA721710.1450050005638782Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC715790.14381556214216012No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG707970.14224437828103928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG701950.14103484799408947No Hit
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA691830.13900155123263896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCGGTTGGCCCCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGT665920.13379574895109914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG651940.13098690619170408No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG646460.12988587197700557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT646270.12984769743306532Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC645230.12963874203465536No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT633280.1272377641394643Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAA633000.12718150691681548Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC611130.12278741599063735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC609870.12253425848871762No Hit
TCTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGA602770.12110773605726519Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCT562390.11299464087669489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA560160.11254659228202742No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG537000.10789331629436005No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG535770.10764618635200984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC535610.1076140393676391TruSeq Adapter, Index 5 (100% over 35bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC530500.10658734505429796No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA519420.10436116638662289No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC517940.10406380678119338No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC508880.10224348379119916Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGAAC505950.10165479213990962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT500870.10063412538613803Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT500630.10058590490958187Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)