FastQCFastQC Report
Tue 4 Jul 2023
SRR12671772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12671772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36312081
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG24064206.627050650167915No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT17222834.743002748864765No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA14351533.9522741756386806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG12654963.485055015161483No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC10369162.8555675451373883No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT7900942.1758433508671673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA7853272.162715488544983No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT7078821.9494393615171766No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG5739691.580655760268876No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT5219641.4374389614299439No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT4204041.1577524295564332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC3645661.0039799151142013No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3562570.9810977233720094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3547150.9768512027718819Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC3344940.9211645016984843No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2864060.7887347464332876Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2863830.7886714066318589No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG2813450.7747972362145811No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA2517040.6931687555995483No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2492330.6863638578025865Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2303730.6344252206311172No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2131050.5868707992802726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA1962730.5405170802521617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1885960.5193753560970521No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1864600.5134930162774202No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1722060.4742388628181348Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1689880.4653767984269478No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1670780.46011684100396233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGAGATCGGAAGAGCACAC1513040.41667675284156813No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1511470.41624438984920753No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1495580.41186843574181276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1459090.40181943854994157No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1400160.3855906798621649Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC1366270.37625769781687807No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG1339090.36877258563066107No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1168680.3218433005808728Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1125590.30997672647844116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1097170.30215013014539155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1072300.2953011698778707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1003090.27624139745667564No Hit
CCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA950930.26187703205442836No Hit
TCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCACAC925590.2548986382796403No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA870040.2396006992824234No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA858370.23638689283602335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC838500.2309148847734725No Hit
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT801340.2206813759861353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT800300.22039496992750152No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA791180.21788340910563622No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA769000.2117752491243892No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC738270.20331250087264346No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA676150.18620524667809593No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCAC666260.18348163521666522No Hit
GCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA666020.18341554151082667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA661830.1822616555630618Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA634490.1747324809062857No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG630120.1735290246791419Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA623710.17176377195237033Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC607840.1673933256537955TruSeq Adapter, Index 5 (97% over 35bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC600810.16545733085360764No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA599360.16505801471416634Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT597040.16441910889106026Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT565740.15579938808794794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCAGATCGGAAGAGCACACGTCTGA555230.15290503455310092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA542870.14950120870241504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA528420.1455218168300517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC522510.1438942593237771No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA515530.14197203404563896No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT502410.13835891145979765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA500280.1377723298204804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG498200.13719951770321287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA494190.13609520203482692Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA488450.13451446090352134Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA487840.134346472734515No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACG487480.13424733217575716No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT483010.13301633690451395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC479920.13216538044184248No Hit
CTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG464140.1278197192829571No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC462530.12737634067295675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC450790.12414325689568714No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA450690.12411571785158773No Hit
CTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT449840.12388163597674283Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTG434720.11971773250891349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA433070.11926333828127338Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACG432810.11919173676661494No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGAGATCGGAAGAGC432450.11909259620785712No Hit
TCTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTG421330.11603025450400378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG419570.11554556732785433No Hit
GTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG415420.1144026969977292No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGT414090.11403642771120719No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGA413330.11382713097605175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC407900.1123317608814543No Hit
GCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT407740.11228769841089527Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCA399020.10988629376542755No Hit
TTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGA390790.10761983043604689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC385630.10619881576051783No Hit
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC379710.10456850434983332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT375190.10332373955654042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
NCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG374750.10320256776250307No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG373660.1029023921818196Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT367820.10129411200641462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG366600.10095813566840192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG365850.10075159283765643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)