FastQCFastQC Report
Wed 30 Aug 2023
SRR12672553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12672553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45023803
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGAAGACGGTCGAACTTG40262428.94247427299733No Hit
CTCTCGCTTCTGGCGCCAAGCGCC21364884.745241089474383No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGTC13349472.964980545956991No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCAG13041172.896505655019857No Hit
TCTATTTTGTTGGTTTTCGGAAC12495772.775369730540088No Hit
CTGAGAAGACGGTCGAACTTG11512002.556869751762196No Hit
GCCGCCGGTGAAATACCACTACTC10959002.4340458312684072No Hit
CGCTGAGAAGACGGTCGAACTTG8468331.8808562217634082No Hit
GCTCTCGCTTCTGGCGCCAAGCGCC8214531.8244860390847037No Hit
CTCTCGCTTCTGGCGCCAAGCG7092631.575306732752007No Hit
GGGCCCGGGGGGGCCGTCGTCACGCGCTCTC6907251.5341329562942518No Hit
GCCGCCGGTGAAATACCACTACTCT6891811.530703659128928No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCC5480861.2173249780788176No Hit
TTCTATTTTGTTGGTTTTCGGAAC5421711.2041874827854946No Hit
CATCGTTTTTTCACTGACCCGGTGAGGCGGGG4991501.1086358031550554No Hit
CTCTCGCTTCTGGCGCCAAGCGC4909151.0903454779241994No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCG4842691.075584396991076No Hit
GGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCC4641821.030970218131063No Hit
CCCGAGGTGGGATCCCGAGGCCTCTCCAG4470450.9929081290623096No Hit
GGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCC4164810.9250240367300825No Hit
GGCCCGGGGGGGCCGTCGTCACGCGCTCTC4153680.9225520109884987No Hit
GCTCTCGCTTCTGGCGCCAAGCGCCCG3592170.7978379791684855No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTC3504970.7784704459549985No Hit
GAGGTGTAGAATAAGTGGGAGGCCC3195050.7096357453411921No Hit
TCTCGCTTCTGGCGCCAAGCGCC3171610.704429610266374No Hit
GCTCTCGCTTCTGGCGCCAAGCGC3161860.7022640890641779No Hit
TCTCGCTTCTGGCGCCAAGCG3028480.6726397590181353No Hit
GCTCTCGCTTCTGGCGCCAAGCG2852550.6335648723409704No Hit
TCTATTTTGTTGGTTTTCGGAACTG2751900.6112100303921462No Hit
TTCTATTTTGTTGGTTTTCGGAACTG2242950.49816982363751017No Hit
GGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCC2080780.46215109816467526No Hit
GAGGTGTAGAATAAGTGGGAGGCCCCC1932940.4293151335972219No Hit
TCTCGCTTCTGGCGCCAAGCGC1860780.41328805565358395No Hit
GCCGCCGGTGAAATACCACTACTCTC1686820.37465071531163197No Hit
CATCGTTTTTTCACTGACCCGGTGAGGCGGGGG1683620.37393998014783425No Hit
GAGGTGTAGAATAAGTGGGAGGCCCC1573260.3494285011863614No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGG1458510.3239419824220535No Hit
GGGAAGGTGGCTCGGGGGGG1442440.32037275927135694No Hit
AGGTGTAGAATAAGTGGGAGGCCC1235440.2743970783631938No Hit
GGGAAGGTGGCTCGGGGGGGGCGG1224890.27205387337004827No Hit
GCTCGTGGGGGGCCCAAGTCCT1207930.2682869770019205No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCC1182950.26273880062952476No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCCC1159130.2574482657540057No Hit
GGGAAGGTGGCTCGGGGGG1154240.2563621735818274No Hit
GGGAAACGGGGTGCGGCCGGAAAGGGGGCCGC1114800.24760236268802083No Hit
GGCCGCCGGTGAAATACCACTACTCT1057690.23491796106161889No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGTCCG1046070.23233710399807855No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGG978870.21741166555832703No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCA956890.2125298034019916No Hit
GGCCGCCGGTGAAATACCACTACTC954370.21197009946050094No Hit
GCGCTGAGAAGACGGTCGAACTTG880370.1955343487976793No Hit
GGGAAGGTGGCTCGGGGGGGGC859420.1908812545221913No Hit
GGGAAGGTGGCTCGGGGGGGG850670.18893783805868197No Hit
CGCTCGTGGGGGGCCCAAGTCCT836550.18580171914842467No Hit
GCCCGGGGGGGCCGTCGTCACGCGCTCTC815540.18113529858861543No Hit
CGTCCCCGCCCCTTGCCTCTCGGCG803620.17848781010346906No Hit
CTCGCTTCTGGCGCCAAGCGCC738920.16411763351043446No Hit
TCTCGCTTCTGGCGCCAAGCGCCCG734780.1631981198922712No Hit
TGAGGTAGTAGTTTGTGCTGTT722630.16049954731722685No Hit
TGAGGTAGTAGATTGTATAGTT717130.15927797125444956No Hit
CTCGCTTCTGGCGCCAAGCG687230.15263703956771488No Hit
AGGTGTAGAATAAGTGGGAGGCCCC664280.14753973581485333No Hit
CGCGGGAAATGTGGCGTACGGAAGAC653970.14524983595899263No Hit
AGGTGTAGAATAAGTGGGAGGCCCCC638080.14172059166125972No Hit
GGGACGCGTGCATTTATCAGATCAAAAC617990.13725850746104232No Hit
GACGCGTGCATTTATCAGATCAAAACCAA604080.13416903054590926No Hit
CGTGGGGGGCCCAAGTCCT597160.13263206575419673No Hit
AGCGCTGAGAAGACGGTCGAACTTG572330.12711720509260402No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTC566680.12586231331902373No Hit
TGAGGTAGTAGGTTGTATAGTT554340.12312154084362886No Hit
TCCCTGGTCTAGTGGTTAGGATTCGG551140.12241080567983117No Hit
TTCTATTTTGTTGGTTTTCGGAACT541700.12031413694662799No Hit
CATCGTTTTTTCACTGACCCGGTGAGG540920.1201408952504523No Hit
CCGGGGGGGCCGTCGTCACGCGCTCTC539630.11985438013754636No Hit
GCTCTCGCTTCTGGCGCCAAGCGCCCGTC535980.11904369784133961No Hit
CCGAGGTGGGATCCCGAGGCCTCTCCAG529680.1176444379876129No Hit
TTTGTTCGTTCGGCTCGCGTGA512940.11392640466199623No Hit
GGTCGCGGGGGCCGGGCCGCCC484770.10766971417318968No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCCCCG480360.106690232275581No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTC474650.1054220142176795No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC465520.10339419795346919No Hit
GGCTGAGACAGGAGGATCGCTTGAGTC464390.10314321959875312No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG463880.10302994618202287No Hit
ACGCTTAACGCACGTTCGTGTGGAACCTG463520.10294998847609563No Hit
GAAACGGGGTGCGGCCGGAAAGGGGGCCGC450690.10010038467874426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)