FastQCFastQC Report
Wed 30 Aug 2023
SRR12672554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12672554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50260297
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length18-44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATGAGAGGTGTAGAATAAGTGGGAGGCCC1579563331.427655511068707No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTG734781314.61951766819046No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCC39221827.803738207118036No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCC25838285.140892820430409No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACC20971844.172645458103839No Hit
CGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGCTC4984610.9917589623475563No Hit
GAGGTGTAGAATAAGTGGGAGGCCC4473180.8900026993473596No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC4350840.8656614185944822No Hit
GAGGTGTAGAATAAGTGGGAGGCCCCC4288280.85321421797408No Hit
GCGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGAC2712300.5396506112966265No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGAC2436340.48474444948067064No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTTTGGAACCTG2022590.402423009955552No Hit
GAGGTGTAGAATAAGTGGGAGGCCCC1817270.36157167953066416No Hit
GCCCTCGGGCCCGGGGGGGCCGTCGTCACGCGCTCTC1494920.2974355682776805No Hit
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTG1359230.2704381153975274No Hit
CCCCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC1315430.2617234832496115No Hit
CTCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTG1234180.24555764165102326No Hit
CCCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC1222300.2431939469040543No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCT1219000.24253736502989626No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCAG1121710.22318013759449135No Hit
AAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCC1084440.21576474170059123No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTGG1078070.2144973397192619No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGA1064830.21186305365445812No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCC1049240.20876120170957208No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCAC1042530.20742615189878405No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAAC998750.198715499034954No Hit
AGGTGTAGAATAAGTGGGAGGCCC969120.1928201896618319No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAA927250.1844895584281963No Hit
GACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG756740.15056417195465438No Hit
GTCGGCGGGGGACCGCCCCCCGGTCGGCGACCGGCC718520.14295976006667846No Hit
CGTCGTCGCGCCGTCGGGCCCGGGGGGGCCGTCGTCACGCGCTC703410.13995341093985178No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCGG656870.13069361687218045No Hit
AGGTGTAGAATAAGTGGGAGGCCCCC645830.12849705205681533No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG623200.12399449211372549No Hit
GACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGG617520.12286437543335647No Hit
AGACATGAGAGGTGTAGAATAAGTGGGAGGCCC604780.12032957147069784No Hit
AGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCC600310.11944020147752013No Hit
ACATGAGAGGTGTAGAATAAGTGGGAGGCCC584030.11620106423167376No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCA551710.10977054114901072No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC550510.10953178410386234No Hit
CATCGTTTTTTCACTGACCCGGTGAGGCGGGGG539210.10728348859538177No Hit
CCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC522370.10393293139513281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)