FastQCFastQC Report
Wed 30 Aug 2023
SRR12672613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12672613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43251662
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length18-45
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGAAGACGGTCGAACTTG37197748.600303035753862No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCAG10726772.4800827306936784No Hit
CTCTCGCTTCTGGCGCCAAGG10203882.3591879544420746No Hit
CGCTGAGAAGACGGTCGAACTTG8910502.0601520468739443No Hit
CTGAGAAGACGGTCGAACTTG8537801.9739819477919716No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGTC7994071.8482688595874073No Hit
CTCTCGCTTCTGGCGCCAAGCGCC7503001.7347310260586053No Hit
GCCGCCGGTGAAATACCACTACTC6471931.4963424989310237No Hit
GCTCTCGCTTCTGGCGCCAAGG4629251.070305691374357No Hit
GGGAAGGTGGCTCGGGGGGG4344171.004393773353727No Hit
TCTATTTTGTTGGTTTTCGGAAC4251740.9830234962994023No Hit
TCTCGCTTCTGGCGCCAAGG3832280.8860422519717278No Hit
GGGAAGGTGGCTCGGGGGGGGCGG3800090.8785997634033115No Hit
CCCGAGGTGGGATCCCGAGGCCTCTCCAG3476980.8038951196834933No Hit
GGGAAGGTGGCTCGGGGGG3300310.7630481344277591No Hit
CATCGTTTTTTCACTGACCCGGTGAGG3031030.700789255219834No Hit
CTCTCGCTTCTGGCGCCAAGCGC2584700.5975955328606795No Hit
GCCGCCGGTGAAATACCACTACTCT2518580.5823082590444733No Hit
GGGAAGGTGGCTCGGGGGGGG2344080.5419629886130156No Hit
GCTCTCGCTTCTGGCGCCAAGCGCC2263470.5233255545185755No Hit
GCTCGTGGGGGGCCCAAGTCCT2080380.4809942332389447No Hit
GGCCCGGGGGGGCCGTCGTCACGCGCTCTC2058730.47598864524558615No Hit
CGTCCCCGCCCCTTGCCTCTCGGCGCC2029690.46927445238982957No Hit
GGGCCCGGGGGGGCCGTCGTCACGCGCTCTC1972370.4560217824693072No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGTC1964030.45409353286817045No Hit
CGCTCGTGGGGGGCCCAAGTCCT1911160.4418697251448973No Hit
CTCTCGCTTCTGGCGCCAAGCG1813660.41932723880067313No Hit
TTCTATTTTGTTGGTTTTCGGAAC1806330.41763250623756376No Hit
GAGGTGTAGAATAAGTGGGAGGCCC1791370.4141736796149013No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCG1674870.3872382984959052No Hit
CGTCCCCGCCCCTTGCCTCTCGG1453740.33611193946720475No Hit
CGTGGGGGGCCCAAGTCCT1338330.3094285717852877No Hit
GCTCTCGCTTCTGGCGCCAAGCGCCCG1322530.30577553297258264No Hit
TCTATTTTGTTGGTTTTCGGAACTG1315110.3040599919605402No Hit
GGTTCTCCTCCGCGTCGG1277820.29543835795257994No Hit
GCGCTGAGAAGACGGTCGAACTTG1256190.2904373940589844No Hit
CGTGGATCGCCCCAGCTGCGGCGGG1242240.2872120844743492No Hit
GCTCTCGCTTCTGGCGCCAAGCGC1189160.27493972370356545No Hit
GAGGTGTAGAATAAGTGGGAGGCCCCC1118910.2586975732863167No Hit
TTGTGCTTGATCTAACCATGTG1101200.254602932946253No Hit
CGTCGTCGCGCCGTCGGGCCCGGGGGG1085950.2510770568770282No Hit
TCTCGCTTCTGGCGCCAAGCGCC1083320.25046898775820453No Hit
CATCGTTTTTTCACTGACCCGGTGAGCCAAGG1053380.24354671041311662No Hit
TTCTATTTTGTTGGTTTTCGGAACTG1022220.2363423629824907No Hit
TCTCGCTTCTGGCGCCAAGCGC970340.22434744819748198No Hit
GCCGCCGGTGAAATACCACTACTCTC947030.2189580599238013No Hit
GCTCTCGCTTCTGGCGCCAAGCG921040.21294904228188966No Hit
TGGATCGCCCCAGCTGCGGCGGG914860.21152019545514805No Hit
TATTTTGTTGGTTTTCGG888760.20548574526454036No Hit
CGGGGGCCCGGGGGGCGGGG879990.2034580775185009No Hit
CGTCGTCGCGCCGTCGGGCCCGGGGGGG863450.1996339470145679No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG861050.19907905504301776No Hit
TCTCGCTTCTGGCGCCAAGCG805820.18630960354772033No Hit
GAGGTGTAGAATAAGTGGGAGGCCCC800800.18514895450722796No Hit
CTCGCTTCTGGCGCCAAGG789320.18249472124331315No Hit
GGGAAGGTGGCTCGGGGGGGCGG788840.18238374284900313No Hit
TCATCGTTTTTTCACTGACCCGGTGAGG785840.18169012788456546No Hit
GGCCGAAGTGGAGAAGGGTTCCATGTG785000.1814959156945229No Hit
GCCGAAGTGGAGAAGGGTTCCATGTG756850.17498749527821614No Hit
GGGACGCGTGCATTTATCAGATCAAAAC725340.16770222610173918No Hit
CCCCGAGGTGGGATCCCGAGGCC689450.15940427907718321No Hit
GGGAAGGTGGCTCGGGGAGG684250.1582020131388246No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG675960.15628532378709514No Hit
TCTGGTTTAGATGTTGTGTGG652330.15082194991720782No Hit
CGTCCCCGCCCCTTGCCTCTCGGCG641000.14820239740151486No Hit
ATCGTTTTTTCACTGACCCGGTGAGCCAAGG636510.14716428700473985No Hit
AGCGCTGAGAAGACGGTCGAACTTG635430.14691458561754228No Hit
CCGTCCCCGCCCCTTGCCTCTCGGCGCC621980.14380487852698007No Hit
TTTGTTCGTTCGGCTCGCGTGA613450.14183269997809564No Hit
GGCCGCCGGTGAAATACCACTACTC592970.13709762182086782No Hit
TCACACAACATGTAAACCAAGG586820.1356757111437706No Hit
TCTATTTTGTTGGTTTTCGG582940.13477863578976457No Hit
CGGTGCGGAGAGCCATTCGTCCC573130.1325105148560534No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCA546200.1262841645252846No Hit
TGAGGTAGTAGGTTGTATAGTT542650.12546338681736668No Hit
GTCCGGTGCGGAGAGCCATTCGTCCC538370.12447382946810229No Hit
CCGTCCCCGCCCCTTGCCTCTCGG529130.12233749537763428No Hit
GGGAAGGTGGCTCGGGGGGGGCG526080.1216323201637893No Hit
GGGAAGGTGGCTCGGGGGGGGC521620.12060114591665863No Hit
GGCTGAGACAGGAGGATCGCTTGAGTC496130.11470773076881993No Hit
AGGTGTAGAATAAGTGGGAGGCCC496010.11467998617024243No Hit
CCCGCGTCCCGCCGTCGCCGTC495000.11444646913221508No Hit
AAGGCCGAAGTGGAGAAGGGTTCCATGTG491990.11375054211789595No Hit
TGGATCGCCCCAGCTGCGGCAGG480620.1111217414026772No Hit
TCGTGGGGGGCCCAAGTCCT475510.10994028391325171No Hit
GCCCGGGGGGGCCGTCGTCACGCGCTCTC464680.10743633389163172No Hit
TTCACAGTGGCTAAGTTCTG462910.1070271010626135No Hit
GGGAAGGTGGCTCGGGGGAGG441150.10199608052055896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)