FastQCFastQC Report
Wed 30 Aug 2023
SRR12672614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12672614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44596401
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length18-44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATGAGAGGTGTAGAATAAGTGGGAGGCCC1504155433.72817909678407No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTG44580259.996378407306903No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCC25990045.827833506116335No Hit
GAGGTGTAGAATAAGTGGGAGGCCC22280874.996113924081004No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCCCC15521583.4804557435027097No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACC14485393.248107397724763No Hit
GAGGTGTAGAATAAGTGGGAGGCCCCC10846642.4321783275740123No Hit
GAGGTGTAGAATAAGTGGGAGGCCCC6780731.5204657434127924No Hit
CGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGCTC4712061.056600957552606No Hit
AGGTGTAGAATAAGTGGGAGGCCC4130570.9262115120007106No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCC3416640.7661246027454099No Hit
GCGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGAC3210240.7198428411297136No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGAC3066450.6876003290041275No Hit
CCCCGAGGTGGGATCCCGAGGCCTCTCCAG2937700.6587302863296076No Hit
TCTATTTTGTTGGTTTTCGGAAC2770630.621267622021786No Hit
AGGTGTAGAATAAGTGGGAGGCCCCC2277600.5107138578290208No Hit
CGGACTGTCCCCAGTGCGCC2238410.5019261531889088No Hit
TTCTATTTTGTTGGTTTTCGGAAC1890480.42390864679865087No Hit
GACATGAGAGGTGTAGAATAAG1725130.38683166383762674No Hit
AGGTGTAGAATAAGTGGGAGGCCCC1597600.3582351858393237No Hit
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTG1314100.29466503362008967No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAAGA1307720.29323442490348045No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCT1305300.29269178021787007No Hit
GCCCTCGGGCCCGGGGGGGCCGTCGTCACGCGCTCTC1146180.25701177097228095No Hit
CGCGCCGCGGCGCGGGAAATGTGGCGTACGGAA1137400.25504300223688453No Hit
CCCCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC1110250.24895506702435474No Hit
CCCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC997220.2236099724728908No Hit
CCCGAGGTGGGATCCCGAGGCCTCTCCAG937410.21019857633803232No Hit
CGGACTGTCCCCAGTGCGCCC904450.20280784541335523No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCAC863750.19368154842808952No Hit
TACCCTGTAGAACCGAATTTGT822560.18444537710565478No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAAC782080.17536841145544457No Hit
CATCGTTTTTTCACTGACCCGGTGAGGCGGGGG743390.16669282348591313No Hit
AGAGGTGTAGAATAAGTGGGAGGCCC726040.16280237501676426No Hit
GTCGGCGGGGGACCGCCCCCCGGTCGGCGACCGGCC711330.15950390256828123No Hit
CTCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTG640150.14354297334441854No Hit
TCTCGCCCGTCACGCCTAACGCACGTTCGTGTGGAACCTG639870.14348018800889337No Hit
GCTGAGAAGACGGTCGAACTTG625500.14025795489640522No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCCA583140.13075943056481173No Hit
TCTCGCCCGTCACGCTTAACGCACGTTCGTGTGGAACCTGG578180.12964723319265156No Hit
GCGCTGAGAAGACGGTCGAACTTG558620.12526122903953618No Hit
GGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCC545140.12223856360068158No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCC535410.12005677319118196No Hit
GACATGAGAGGTGTAGAATAAGTGGGAGGCC510220.11440833532732832No Hit
CCTTCCCGTGGGGGGGACGCGTGCATTTATCAGATCAAAAC495390.11108295487790595No Hit
AGAGGTGTAGAATAAGTGGGAGGCCCCC479890.1076073380899055No Hit
ACATGAGAGGTGTAGAATAAGTGGGAGGCCC453000.10157770354607763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)