FastQCFastQC Report
Wed 28 Jun 2023
SRR1272131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86223360
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGCCAACGGGCTGTAGGCACCATCAATAGATC27607093.2018109709480123No Hit
ACGATGAGGCAGCGAAAAGAAATCTGTAGGCACCAT11714981.3586782050711084No Hit
ACGATGAGGCAGCGAAAAGAAATAGAGCTGTAGGCA11119641.2896319512484784No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10664561.2368527508090614No Hit
TGCTTCACTTATCGAATGAAGAGACCCTGTAGGCAC6456210.7487773614946112No Hit
TAGCCGTGCGGAACTCTGTAGGCACCATCAATAGAT6088980.7061868152667676No Hit
CCCGTGGGCGCCCCTGTAGGCACCATCAATAGATCG5839630.6772677381164455No Hit
TTGCTTCACTTATCGAATGAAGAGACCCTGTAGGCA4345890.5040269829429055No Hit
TTGCGCCAACGGGCTGTAGGCACCATCAATAGATCG4294770.49809819519907367No Hit
TCCCGTGGGCGCCCCTGTAGGCACCATCAATAGATC4262030.49430108035687775No Hit
TATCGAATGAAGAGACCCTGTAGGCACCATCAATAG4180160.48480597369436773No Hit
TAGCGAAAAGAAATAGAGCTGTAGGCACCATCAATA4003030.4642628169442713No Hit
TTACTGCGCCAACGGGCTGTAGGCACCATCAATAGA3325660.38570290000296903No Hit
ACGATGAGGCAGCCTGTAGGCACCATCAATAGATCG3299460.3826642803064042No Hit
ATTCCGTGCGAAAGCCGAGCTGTAGGCACCATCAAT3066500.3556460801342003No Hit
AGCCGTGCGGAACTCTGTAGGCACCATCAATAGATC2913040.3378481191176034No Hit
AGCGAAAAGAAATAGAGCTGTAGGCACCATCAATAG2825360.3276791811407025No Hit
TTATCGAATGAAGAGACCCTGTAGGCACCATCAATA2732280.31688396276832637No Hit
TGTTGCTTCACTTATCGAATGAAGAGACCCTGTAGG2602070.30178248678780323No Hit
TACTGCGCCAACGGGCTGTAGGCACCATCAATAGAT2484200.2881121774887919No Hit
TCGATGAGGCAGCGAAAAGAAATCTGTAGGCACCAT2465900.28598978281523707No Hit
TTTGCTTCACTTATCGAATGAAGAGACCCTGTAGGC2438080.2827632790000297No Hit
TGCTTCACTTATCGAATGAAGAGACTGTAGGCACCA2431890.2820453761022535No Hit
TTTCTGCGCCAACGGGCTGTAGGCACCATCAATAGA2425270.2812776027285413No Hit
ACTTATCGAATGAAGAGACCCTGTAGGCACCATCAA2421850.2808809584780737No Hit
TCGATGAGGCAGCGAAAAGAAATAGAGCTGTAGGCA2416050.28020828694219296No Hit
CCCCGTGGGCGCCCCTGTAGGCACCATCAATAGATC2407600.2792282741011253No Hit
TGTCCCGAGGCGCTGAACCTTGATGCTTGGACTGTA2373970.27532793897152696No Hit
TCTGCGCCAACGGGCTGTAGGCACCATCAATAGATC2065100.23950586012885605No Hit
TGAATGAAGAGACCCTGTAGGCACCATCAATAGATC2005120.2325495086250408No Hit
TGTCCCGAGGCGCTGAACCTTGATGCTTGGCTGTAG1985900.23032041432855319No Hit
TCTTCACTTATCGAATGAAGAGACCCTGTAGGCACC1917240.22235737507793712No Hit
TTGCTTCACTTATCGAATGAAGAGACTGTAGGCACC1858330.21552511987470682No Hit
TGCTTCACTTATCGAATGAAGAGACCTGTAGGCACC1825640.2117338039250616No Hit
TCGTGTTGACTCAATTCCTGTAGGCACCATCAATAG1753880.20341123333630237No Hit
AAATGGGGGATACTCCTGTAGGCACCATCAATAGAT1730800.20073446453490099No Hit
TCGTCTAGGCTGGGCTGTAGGCACCATCAATAGATC1581450.18341317248597133No Hit
TAGCCGTGCGGAACTACTGCGCCAACGGGCTGTAGG1514620.17566237270271073No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA1453560.1685807651198005No Hit
TTGGATTGAGGTTCCAATCCTGTAGGCACCATCAAT1441040.16712872242510612No Hit
ATTCCGTGCGAAAGCCGAGCCTGTAGGCACCATCAA1434310.16634819148778243No Hit
ATCGAATGAAGAGACCCTGTAGGCACCATCAATAGA1408630.16336988027374483No Hit
AAAATTGCCCAATGTCGAACTGTAGGCACCATCAAT1319190.1529968212790594No Hit
TCGCCCAGCCGTGCGGAACTCTGTAGGCACCATCAA1309480.1518706763457142No Hit
TTGCTTCACTTATCGAATGAAGAGACCTGTAGGCAC1294620.15014724547964728No Hit
TTTCAATGGGGGATACTCCTGTAGGCACCATCAATA1271670.1474855537988777No Hit
GCTTCACTTATCGAATGAAGAGACCCTGTAGGCACC1256810.14576212293281077No Hit
TAGCCGTGCGGAACTTCTGCGCCAACGGGCTGTAGG1249570.14492244329146997No Hit
ACCCGTGGGCGCCCCTGTAGGCACCATCAATAGATC1243140.14417670570945043No Hit
AAAAAAAGTGCGACCAAAGCCTGTAGGCACCATCAA1229740.14262260250586384No Hit
TAGCGAAAAGAAATCTGTAGGCACCATCAATAGATC1225210.14209722284077075No Hit
ACGCGCCTCCTCCTGTAGGCACCATCAATAGATCGG1197300.1388602810189721No Hit
ATGGGATAACGAAGCCTGTAGGCACCATCAATAGAT1197280.13885796146195184No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1186950.137659910260978No Hit
TCGCCCAGCCGTGCGGAACTACTGCGCCAACGGGCT1183490.13725862689646984No Hit
TGAACCTTGATGCTTGGCTGTAGGCACCATCAATAG1152970.13371898288352485No Hit
ATGGCTCATTACATCCTGTAGGCACCATCAATAGAT1139950.13220895126332355No Hit
TGCGCCAACGGGCTGTAGGCACCATCAATAGATCGG1135380.13167893248418988No Hit
ACCTGCAGCTGGATCCTGTAGGCACCATCAATAGAT1051610.12196346790475342No Hit
TATCGAATGAAGAGACCTGTAGGCACCATCAATAGA1049340.12170019818295182No Hit
TGTGTTGACTCAATTCCTGTAGGCACCATCAATAGA992880.11515208871470561No Hit
TTTACGATGAGGCAGCGAAAAGAAATAGAGCTGTAG980520.1137186024761735No Hit
TCGTCCCGAGGCGCTGAACCTTGATGCTTGGACTGT970220.11252403061073007No Hit
GTCTAGGCTGGGCTGTAGGCACCATCAATAGATCGG958890.11121000155874233No Hit
CGCATGGCTCATTACATCCTGTAGGCACCATCAATA951920.11040163593717527No Hit
TCAATGGGGGATACTCCTGTAGGCACCATCAATAGA949910.11016852045663728No Hit
TCGAATGAAGAGACCCTGTAGGCACCATCAATAGAT934730.1084079766782459No Hit
TGAACCTTGATGCTTGGACTGTAGGCACCATCAATA934510.10838246155102282No Hit
AGCCGTGCGGAACTACTGCGCCAACGGGCTGTAGGC923600.10711714319646091No Hit
GTCCCGAGGCGCTGAACCTTGATGCTTGGACTGTAG904990.10495879538909177No Hit
CTGCGCCAACGGGCTGTAGGCACCATCAATAGATCG903470.1047825090555506No Hit
TTATCGAATGAAGAGACTGTAGGCACCATCAATAGA899980.10437774635551202No Hit
ACGGGAATATCCTCAGCCTGTAGGCACCATCAATAG895800.1038929589382738No Hit
TGAGGGCAGCAGGCGCGCCTGTAGGCACCATCAATA895380.10384424824084795No Hit
TTTACGATGAGGCAGCGAAAAGAAATCTGTAGGCAC890090.1032307254089843No Hit
GGAACTACTGCGCCAACGGGCTGTAGGCACCATCAA875110.10149337720079571No Hit
ACTGCGCCAACGGGGCTGTAGGCACCATCAATAGAT866060.10044377764911969No Hit
TCTTGATGCTTGGACTGTAGGCACCATCAATAGATC863440.1001399156794632No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)