FastQCFastQC Report
Wed 28 Jun 2023
SRR1272132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64636189
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23480163.6326646671572793No Hit
ACGATGAGGCAGCGAAAAGAAATAGAGCTGTAGGCA2504130.3874191902000905No Hit
ACTGCGCCAACGGGCTGTAGGCACCATCAATAGATC2457740.38024209626591693No Hit
TGTCCCGAGGCGCTGAACCTTGATGCTTGGCTGTAG2043850.31620830863032473No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA1842600.28507250017478597No Hit
TCGTCCCGAGGCGCTGAACCTTGATGCTTGGCTGTA1574590.24360811247705214No Hit
CGTTGCTTCACTTATCGAATGAAGAGACCCTGTAGG1467000.22696263853056062No Hit
TGTCCCGAGGCGCTGAACCTTGATGCTTGGACTGTA1437130.22234138835134604No Hit
TCGCCCAGCCGTGCGGAACTCTGTAGGCACCATCAA1236480.19129840715083002No Hit
ACGATGAGGCAGCGAAAAGAAATCTGTAGGCACCAT1167770.18066813932981104No Hit
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGCTGTA1125690.174157854510884No Hit
TGTTGCTTCACTTATCGAATGAAGAGACCCTGTAGG1015480.15710703488412658No Hit
ACTTATCGAATGAAGAGACCCTGTAGGCACCATCAA1014670.15698171808984593No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG957950.1482064482483644No Hit
GTCTAGGCTGGGCTGTAGGCACCATCAATAGATCGG934720.14461248635806792No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCT889750.13765508359411474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTTCCGTTTGCGCCACTGTAGGCACCATCAATA862310.13340978379774215No Hit
TCTGCGCCAACGGGCTGTAGGCACCATCAATAGATC853820.1320962781391706No Hit
TGAGGGCAGCAGGCGCGCAAATTGCCCAATGTCGAA848820.1313227176806479No Hit
TCGTTGCTTCACTTATCGAATGAAGAGACCCTGTAG833190.12890456768730596No Hit
TCGCCCAGCCGTGCGGAACTACTGCGCCAACGGGCT825050.12764521126083098No Hit
TCGTCCCGAGGCGCTGAACCTTGATGCTTGGACTGT824510.12756166673131053No Hit
ATGATTCTCCCTTCTGTAGGCACCATCAATAGATCG749920.11602169181106887No Hit
GTCCCGAGGCGCTGAACCTTGATGCTTGGCTGTAGG704740.10903179950785774No Hit
ACGTTGCTTCACTTATCGAATGAAGAGACCCTGTAG702150.10863109519034299No Hit
ACCTGCAGCTGGATCCTGTAGGCACCATCAATAGAT700000.10829846419317822No Hit
GGTACGCTCATCACCCTGTAGGCACCATCAATAGAT690960.10689986688416916No Hit
TTTACGATGAGGCAGCGAAAAGAAATAGAGCTGTAG689070.1066074610308476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)