FastQCFastQC Report
Wed 28 Jun 2023
SRR1272133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68616219
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG59066398.608225702439244No Hit
TTTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTG17776272.590680492027694No Hit
TGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGG15419972.2472777172405842No Hit
ACGGGAATATCCTCAGCACGTTTCTTCTGTAGGCAC13171951.9196554680461189No Hit
TACGGGAATATCCTCAGCACGTTTCTTCTGTAGGCA9914491.4449193127356668No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAG8526181.242589598240614No Hit
TTTACGGGAATATCCTCAGCACGTTTCTTCTGTAGG7483811.0906765352372447No Hit
CTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG7280241.061008622465776No Hit
TCCCGTGGGCGCCCAGCCGTGCGGAACTACTGCGCC6702840.9768594215312272No Hit
CCCGTGGGCGCCCAGCCGTGCGGAACTACTGCGCCA6687850.9746748068412222No Hit
TTGCGCAGCCTTCGGGCTGTGCGCCGTCTAGGCTGG6505850.9481504657084064No Hit
TTTTACGGGAATATCCTCAGCACGTTTCTTCTGTAG6170450.899269894192217No Hit
TCGGGAATATCCTCAGCACGTTTCTTCTGTAGGCAC4232120.6167812889835856No Hit
CCCCGTGGGCGCCCAGCCGTGCGGAACTACTGCGCC3793040.5527905873099769No Hit
GTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG3741400.5452646698588857No Hit
AAATTGCAAAGCAAACCCGTTGCTGAACACCTGTAG3630030.5290338134195357No Hit
ACCCGTGGGCGCCCAGCCGTGCGGAACTACTGCGCC3577940.5214423138063028No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATCTGTAGG3324280.484474377697786No Hit
GCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGG2700160.3935162909515606No Hit
TCCCGTGGGCGCCCAGCCGTGCGGAACTTCTGCGCC2248690.3277198937469872No Hit
TTTTACACCCGTGGCGTGCGCAATCTGTAGGCACCA2187600.3188167508909227No Hit
CCCGTGGGCGCCCAGCCGTGCGGAACTTCTGCGCCA2157070.3143673655349619No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAGG2138680.3116872411754428No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGC2078750.30295315455956556No Hit
GCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGC2027550.29549136188923497No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2019620.29433565845416226No Hit
TTTGCGCAGCCTTCGGGCTGTGCGCCGTCTAGGCTG1949350.2840946394904097No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCACTGTAGGC1793570.2613915523383764No Hit
TTTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTA1710720.24931714759742155No Hit
TACCCGTGGGCGCCCAGCCGTGCGGAACTACTGCGC1576560.22976491899094587No Hit
CTTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTG1535580.2237925700919195No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCCTGTAGGCA1530360.22303181701107722No Hit
CTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAG1485980.21656395844253676No Hit
CTTACGGGAATATCCTCAGCACGTTTCTTCTGTAGG1472070.21453674094167155No Hit
ACGGAGCCTACGCGCCTCCTCATGCTGTAGGCACCA1464350.21341164251559827No Hit
TTTTTACGGGAATATCCTCAGCACGTTTCTTCTGTA1416780.20647887928654302No Hit
TTACGGGAATATCCTCAGCACGTTTCTTCTGTAGGC1416120.20638269211540206No Hit
TGCGCAGCCTTCGGGCTGTGCGCCGTCTAGGCTGGG1386390.20204989726991515No Hit
TAGCCGTGCGGAACTACTGCGCCAACGGGCTGTAGG1384890.20183129006277656No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA1329920.19382006461183762No Hit
ATGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG1286620.18750960323243693No Hit
CCCCGTGGGCGCCCAGCCGTGCGGAACTTCTGCGCC1265630.18445055971387758No Hit
AGCACGTTTCTTCTGTAGGCACCATCAATAGATCGG1260090.1836431704288457No Hit
TCGCCCAGCCGTGCGGAACTACTGCGCCAACGGGCT1250440.1822367973962541No Hit
TGCTTCACTTATCGAATGAAGAGACCCTGTAGGCAC1234430.17990352980539484No Hit
TACGGGAATATCCTCAGCACGTTTCTCTGTAGGCAC1214360.1769785653738805No Hit
CCCCACTCTCGTCACCACTGTAGGCACCATCAATAG1184090.1725670719338237No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTA1151940.1678815907941532No Hit
TTTAGCCACGGAGCCTACGCGCCTCCTCCTGTAGGC1147070.16717184606164323No Hit
CCGTGGGCGCCCAGCCGTGCGGAACTACTGCGCCAA1143710.1666821659176528No Hit
TCGTCCAGCGAATGAATGAAATTAGAACCAACGCTG1136540.16563722346753032No Hit
ACTGCGCCAACGGGCTGTAGGCACCATCAATAGATC1093510.1593661113854146No Hit
TTTCAATGGGGGATACTCAAACCCATCCAATCTGTA1087180.1584435889712897No Hit
ACCCTTCCGTTTGCGCCACTGTAGGCACCATCAATA1022400.1490026723856644No Hit
TTGGGGGGGACGCGTGCATTTATCAGACTGTAGGCA1010000.1471955194733187No Hit
ACGGGAATATCCTCAGCACGTTTCTCTGTAGGCACC990620.14437111435708808No Hit
TAATTGCAAAGCAAACCCGTTGCTGAACACCTGTAG969180.14124648867638714No Hit
TTTTACGGGAATATCCTCAGCCTGTAGGCACCATCA967920.14106285862239074No Hit
CCCCACTCTTGTCACCACTGTAGGCACCATCAATAG952080.1387543665150072No Hit
CAATTGCAAAGCAAACCCGTTGCTGAACACCTGTAG928400.13530328740497927No Hit
ACCCTGCTGTCGACGCCACTGTAGGCACCATCAATA925940.134944771585272No Hit
TTTTACACCCGTGGCGTGCGCAATTCTGTAGGCACC901300.13135378386267538No Hit
TCTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTG885910.12911087391743345No Hit
ACCCGTGGGCGCCCAGCCGTGCGGAACTTCTGCGCC872770.12719587478289934No Hit
TTTACGGGAATATCCTCAGCACGTTTCTCTGTAGGC853680.12441373372671553No Hit
TTTGCAAAGCAAACCCGTTGCTGAACACCTGTAGGC838790.12224369285051978No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATCTGTAGGC838120.12214604829799788No Hit
AGCCGTGCGGAACTACTGCGCCAACGGGCTGTAGGC821790.11976614450294908No Hit
GTTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTG805210.11734980617337717No Hit
AAGTCCTTGGGAGATTATGGGGCCGCTGTAGGCACC798310.11634421302053966No Hit
TCGTCCAGCGAATGAATGAAATTAGAACCCTGTAGG780930.11381128418049381No Hit
CCGGGAATATCCTCAGCACGTTTCTTCTGTAGGCAC777690.11333909261307448No Hit
TCAGCACGTTTCTTCTGTAGGCACCATCAATAGATC761330.11095481667388288No Hit
TTTAGCCACGGAGCCTACGCGCCTCCTCATCTGTAG745030.10857928502297684No Hit
TCGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAG736940.10740026348580937No Hit
TTTTTACACCCGTGGCGTGCGCAATCTGTAGGCACC731320.10658121514973012No Hit
TTTACGGGAATATCCTCAGCCTGTAGGCACCATCAA724510.10558873842932091No Hit
ATTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTG698180.10175145325334817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)