FastQCFastQC Report
Tue 27 Jun 2023
SRR1272134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45749193
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT717014315.67272017235364No Hit
ACGGAGCCTACGCGCCTCCTCATGCTGTAGGCACCA4709081.0293252604477636No Hit
ACGTTTCTTCTGTAGGCACCATCAATAGATCGGAAG3969370.8676371624741009No Hit
AGGACAGCAGTCCCTCCGGCGGGGACTGTAGGCACC3546470.7751983734445327No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2664810.5824824057552228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCCTCCTCATGCTGTAGGCACCATCAATAGAT2073990.45333914414621473No Hit
TTTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTA2044630.4469215446051693No Hit
AAGAAACACGGGAGCGGTTCCTCCTCTGTAGGCACC1989780.43493226208383606No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAG1708830.3735213427699151No Hit
ACGGGAGCGGTTCCTCCTCTGTAGGCACCATCAATA1655220.3618031032809694No Hit
TAGGCTGGGCCTGTAGGCACCATCAATAGATCGGAA1474660.32233574043590235No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1411380.3085038024605155No Hit
ACGGGAGCGGTTCCTCCCTGTAGGCACCATCAATAG1265120.27653383962423117No Hit
AAGAAACACGGGAGCGGTTCCTCCCTGTAGGCACCA1170980.2559564274718463No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCTCTG1164790.2546033981408153No Hit
TCCCCGGCACCGGCTGTAGGCACCATCAATAGATCG1128370.2466426019798863No Hit
CACACAGGACAGCAGTCCCTCCGGCGGGGACTGTAG1125870.24609614425329862No Hit
AGAGCATTCGGCTCTGTAGGCACCATCAATAGATCG1115230.24377042016894157No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCTCTGT1079790.23602383543683492No Hit
ATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1063360.2324325152577008No Hit
ATCACCCCCGGTCTGTAGGCACCATCAATAGATCGG1033880.22598868574577918No Hit
TCACGGAGCCTACGCGCCTCCTCATGCTGTAGGCAC1033630.22593403997312042No Hit
TCCCCGGCACCGGACTGTAGGCACCATCAATAGATC1003850.2194246355340082No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGT985770.21547265325532627No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTC970070.2120408987323558No Hit
TAGGCTGGGCACTGTAGGCACCATCAATAGATCGGA943250.20617850024152337No Hit
ACAGGACAGCAGTCCCTCCGGCGGGGACTGTAGGCA932280.20378064373725674No Hit
AGCACGTTTCTTCTGTAGGCACCATCAATAGATCGG848420.18545026575660034No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAGG838240.1832250898939354No Hit
ACCCCTACTCGCTGTAGGCACCATCAATAGATCGGA837830.183135470826775No Hit
AAATTTACGTGCCTATTCTATCCTGTAGGCACCATC821310.17952447816948378No Hit
GCGCCTCCTCATGCTGTAGGCACCATCAATAGATCG813550.17782827338615567No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA787480.17212981221329957No Hit
TCCTCATGCTGTAGGCACCATCAATAGATCGGAAGA738830.16149574485390378No Hit
AGAGCATGTGACTGTAGGCACCATCAATAGATCGGA724220.15830224589972547No Hit
ACGTGCCTATTCTATCCTGTAGGCACCATCAATAGA709290.155038800356544No Hit
ATGATTCTCCCTTCTGTAGGCACCATCAATAGATCG671100.14669111212519093No Hit
ACCCCCGGTCTGTAGGCACCATCAATAGATCGGAAG662250.14475665177307062No Hit
ACCCGGGTGGGTCCCTGTAGGCACCATCAATAGATC659590.14417522075198136No Hit
TACCCAACTTCCTGTAGGCACCATCAATAGATCGGA646630.14134238389735093No Hit
GCCCCACGGGGGGTCTGTAGGCACCATCAATAGATC641960.14032160086408518No Hit
GCCTCCTCATGCTGTAGGCACCATCAATAGATCGGA636030.13902540313661926No Hit
CCCCTGTTGGGAGCCTGTAGGCACCATCAATAGATC625110.13663847578688437No Hit
AGATACAAGAAACACGGGAGCGGTTCCTCCTCTGTA623500.13628655701096193No Hit
ACGAAGCCTACGCGCCTCCTCATGCTGTAGGCACCA603660.13194986849276227No Hit
AGAATTCTCGCCTGTAGGCACCATCAATAGATCGGA590650.1291061024836001No Hit
CCCCTGTTGGGAGCTGTAGGCACCATCAATAGATCG566720.12387540912470302No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCCTGT564290.12334425221445984No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCCTGTA555180.12135296025877441No Hit
AGGACAGCAGTCCCTCCGGCGGGGCTGTAGGCACCA553900.12107317390276152No Hit
TCACACAGGACAGCAGTCCCTCCGGCGGGGACTGTA539040.11782502917592448No Hit
TTCCCGGACTGCGCCTGTAGGCACCATCAATAGATC536360.11723922649302251No Hit
TATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC527230.11524356287552438No Hit
TGCGCCTCCTCATGCTGTAGGCACCATCAATAGATC523990.11453535366186676No Hit
ACACAGGACAGCAGTCCCTCCGGCGGGGACTGTAGG510140.11150797785657116No Hit
TTTTACGTGCCTATTCTATCCTGTAGGCACCATCAA501150.10954291587176193No Hit
ACCCCTACTCGTCCCTGTAGGCACCATCAATAGATC500650.10943362432644439No Hit
CCTACGCGCCTCCTCATGCTGTAGGCACCATCAATA497800.10881066251813448No Hit
AGCAGTCCCTCCGGCGGGGACTGTAGGCACCATCAA492820.10772211872677187No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGG486250.10628602782129948No Hit
ACGGGAGCGGTTCCTCTGTAGGCACCATCAATAGAT483510.10568711015295942No Hit
CCCCACTCTCGTCACCACTGTAGGCACCATCAATAG474290.10367177405730414No Hit
AGTGCACTGGTTCTGTAGGCACCATCAATAGATCGG472380.10325428035419117No Hit
TATCCTCAGCCTGTAGGCACCATCAATAGATCGGAA470650.10287613160739251No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACG466470.10196245428853795No Hit
AGAATTCTCGCCCTGTAGGCACCATCAATAGATCGG464980.1016367654834917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)