FastQCFastQC Report
Wed 28 Jun 2023
SRR1272135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48551383
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT803710316.553808570190473No Hit
ACGGAGCCTACGCGCCTCCTCATGCTGTAGGCACCA4408310.9079679563401933No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA2893290.595923292236598No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2798300.5763584530640455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCCTCCTCATGCTGTAGGCACCATCAATAGAT2299810.473685785634572No Hit
AGGACAGCAGTCCCTCCGGCGGGGACTGTAGGCACC1986720.4091994660584643No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAG1879180.3870497365646618No Hit
TTTAGCCACGGAGCCTACGCGCCTCCTCATGCTGTA1850300.3811013993154428No Hit
CCCCACTCTCGTCACCACTGTAGGCACCATCAATAG1632320.3362046349946406No Hit
TTACGTGCCTATTCTATCCTGTAGGCACCATCAATA1567140.3227796827126428No Hit
TTTACGTGCCTATTCTATCCTGTAGGCACCATCAAT1504280.30983257469720277No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1462690.3012663923497298No Hit
TTTTACGTGCCTATTCTATCCTGTAGGCACCATCAA1424030.29330369435614223No Hit
TACGTGCCTATTCTATCCTGTAGGCACCATCAATAG1404940.28937177752485443No Hit
CCTACGCGCCTCCTCATGCTGTAGGCACCATCAATA1307060.2692116926926675No Hit
TTACGTGCCTATTCTATCACCCCCGGTCTGTAGGCA1295040.2667359650702432No Hit
ACGGGAGCGGTTCCTCCTCTGTAGGCACCATCAATA1239030.2551997334452862No Hit
ACCCTGCTGTCGACGCCACTGTAGGCACCATCAATA1176430.24230617694247764No Hit
ACGTGCCTATTCTATCACCCCCGGTCTGTAGGCACC1077610.22195248279539226No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1051770.2166302863092489No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTC974760.20076874020251906No Hit
ACGTGCCTATTCTATCCTGTAGGCACCATCAATAGA964500.1986555151271386No Hit
CACACAGGACAGCAGTCCCTCCGGCGGGGACTGTAG959750.19767717018483286No Hit
TCTAGGCTGTAGGCACCATCAATAGATCGGAAGAGC871090.17941610437750044No Hit
CCCCACTCTTGTCACCACTGTAGGCACCATCAATAG864350.17802788439620762No Hit
TTTACGTGCCTATTCTATCACCCCCGGTCTGTAGGC856600.17643163738507717No Hit
ACCCTTCCGTTTGCGCCACTGTAGGCACCATCAATA828050.17055126936342885No Hit
TCACGGAGCCTACGCGCCTCCTCATGCTGTAGGCAC812650.16737937207679543No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGC748630.1541933419280765No Hit
TCCCCGGCACCGGACTGTAGGCACCATCAATAGATC746100.153672244516701No Hit
AAATTTACGTGCCTATTCTATCCTGTAGGCACCATC698300.1438270048867609No Hit
TCACAGGACAGCAGTCCCTCCGGCGGGGACTGTAGG668300.13764798419851398No Hit
ACGCGCCTCCTCATCTGTAGGCACCATCAATAGATC667560.13749556835487053No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATGCTGTAGG662550.13646367189993333No Hit
TACGCGCCTCCTCATGCTGTAGGCACCATCAATAGA649050.1336831125902222No Hit
ATTTACGTGCCTATTCTATCCTGTAGGCACCATCAA646680.13319496995585067No Hit
TATTCTATCACCCCCGGTCTGTAGGCACCATCAATA621260.1279592797593428No Hit
TTTTACGTGCCTATTCTATCACCCCCGGTCTGTAGG572800.11797810167426127No Hit
ACAGGACAGCAGTCCCTCCGGCGGGGACTGTAGGCA557380.11480208504050235No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCTCTGT538030.11081661669658308No Hit
TACGTGCCTATTCTATCACCCCCGGTCTGTAGGCAC537270.11066008150581415No Hit
CCCCACCACCGTCACCACTGTAGGCACCATCAATAG536040.11040674165759604No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCTCTG525030.10813904106500942No Hit
ATCCAACGGGAGGACTGTAGGCACCATCAATAGATC521350.10738108119391779No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGG509210.10488063748874055No Hit
GTCTAGGCTGTAGGCACCATCAATAGATCGGAAGAG491500.10123295560911211No Hit
TTGACCCACGCCCTCGTGGCCCGTGAACACACTGTA490450.10101668988502345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)