FastQCFastQC Report
Wed 28 Jun 2023
SRR1272136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86171690
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTACACCCGTGGCGTGCGCAATTCTGTAGGCACC9743511.130708937007038No Hit
TTTTACACCCGTGGCGTGCGCAATCTGTAGGCACCA9650811.1199513436489408No Hit
ACGGGAGCGGTTCCTCCCTGTAGGCACCATCAATAG9323551.0819736737204526No Hit
ACGGGAGCGGTTCCTCCTCTGTAGGCACCATCAATA8592370.9971221406937708No Hit
ACGCGCCTCCTCATGCTGTAGGCACCATCAATAGAT7936540.921014778751583No Hit
ACGGGAGCGGTTCCTCTGTAGGCACCATCAATAGAT7919590.9190477754352966No Hit
TTTTACACCCGTGGCGTGCGCCTGTAGGCACCATCA5796170.6726304195728319No Hit
ATCCTGCTGGTGACGCCACTGTAGGCACCATCAATA4760930.5524935161420184No Hit
AAGAAACACGGGAGCGGTTCCTCCCTGTAGGCACCA4216270.48928714291201675No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCTCTGT4153470.48199936661332743No Hit
ACGGGAATATCCTCAGCACGTTTCTTCTGTAGGCAC4126730.4788962593167199No Hit
AAGAAACACGGGAGCGGTTCCTCTGTAGGCACCATC3953840.45883282549059906No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCTGTAGG3901890.4528041634091196No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3871750.44930649497532194No Hit
CAGATACAAGAAACCTGTAGGCACCATCAATAGATC3848660.4466269606642274No Hit
AAGAAACACGGGAGCGGTTCCTCCTCTGTAGGCACC3801640.44117041223167375No Hit
ACGCGCCTCCTCATCTGTAGGCACCATCAATAGATC3197350.37104413293971605No Hit
TTTTTACACCCGTGGCGTGCGCCTGTAGGCACCATC2931870.3402358709687602No Hit
TTTACACCCGTGGCGTGCGCAATTCTGTAGGCACCA2834060.32888527543094487No Hit
TCGTCCAGCGAATGAATGAAATTCTGTAGGCACCAT2658230.3084806622685478No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCCTGTA2611250.30302875573172583No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCTCTG2534950.29417433962360495No Hit
ACGTCCAGCGAATGAATGAAATTCTGTAGGCACCAT2472850.28696779650021953No Hit
AGCACGTTTCTTCTGTAGGCACCATCAATAGATCGG2417200.2805097590635625No Hit
TACGGGAATATCCTCAGCACGTTTCTTCTGTAGGCA2411270.27982159802134554No Hit
TCAGATACAAGAAACCTGTAGGCACCATCAATAGAT2183560.2533964460949994No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCTGTAG2108700.2447091382332179No Hit
ACCTGCAGCTGGATCCTGTAGGCACCATCAATAGAT2055040.23848203510921048No Hit
ACCCTGCTGTCGACGCCACTGTAGGCACCATCAATA1862110.21609301152153335No Hit
TTTTTACACCCGTGGCGTGCGCAATCTGTAGGCACC1850440.2147387384418247No Hit
ACAAGAAACACGGGAGCGGTTCCTCTGTAGGCACCA1822260.21146852289887783No Hit
CGTGGGGCTCGAGGGGCACTGTAGGCACCATCAATA1791920.20794764498642185No Hit
AGATACAAGAAACACGGGAGCGGTTCCTCTGTAGGC1712260.19870330963684246No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCCTGT1625850.18867565438254721No Hit
ACACCCGTGGCGTGCGCCTGTAGGCACCATCAATAG1619920.18798749334033021No Hit
TTTACACCCGTGGCGTGCGCAATCTGTAGGCACCAT1618410.18781226177646046No Hit
ACAAGAAACACGGGAGCGGTTCCTCCTCTGTAGGCA1526740.17717419723345335No Hit
TTTACACCCGTGGCGTGCGCCTGTAGGCACCATCAA1517320.17608103078864995No Hit
CCTGCAGCTGGATCCTGTAGGCACCATCAATAGATC1490130.17292570216506142No Hit
AGCGAATGAATGAAATTCTGTAGGCACCATCAATAG1476180.17130684102864874No Hit
ACAAGAAACACGGGAGCGGTTCCTCCCTGTAGGCAC1441370.167267231268181No Hit
ACCCGTGGCGTGCGCAATCTGTAGGCACCATCAATA1429190.1658537740178938No Hit
TTTACGGGAATATCCTCAGCACGTTTCTTCTGTAGG1424540.16531415363908958No Hit
ACCCGTGGCGTGCGCAATTCTGTAGGCACCATCAAT1418750.16464223923193338No Hit
TTTTACGGGAATATCCTCAGCACGTTTCTTCTGTAG1398500.16229227951778594No Hit
GGCTTGGGCCTCTCGACTTCTCGCGTTCTGTAGGCA1361830.15803682160579652No Hit
TACGCGCCTCCTCATGCTGTAGGCACCATCAATAGA1355850.15734285819391497No Hit
ACGGGAATATCCTCAGCCTGTAGGCACCATCAATAG1350900.15676842359712337No Hit
CCCCACTCTCGTCACCACTGTAGGCACCATCAATAG1293440.15010034037860925No Hit
TTGACCCACGCCCTCGTGGCCCGTGAACACACTGTA1282130.14878784436048545No Hit
AGATACAAGAAACACGGGAGCGGTTCCTCCTCTGTA1241650.14409024588005642No Hit
TTCCCGGACTGCGCCTGTAGGCACCATCAATAGATC1228080.14251548275309442No Hit
ACACCCGTGGCGTGCGCAATTCTGTAGGCACCATCA1199150.13915823166517913No Hit
ACCCTTCCGTTTGCGCCACTGTAGGCACCATCAATA1194750.13864762313469772No Hit
ACGGGAGCGGTTCCTCCTGTAGGCACCATCAATAGA1153660.13387923574436106No Hit
ACACCCGTGGCGTGCGCAATCTGTAGGCACCATCAA1118570.12980713271377178No Hit
TTAGCCACGGAGCCTCTGTAGGCACCATCAATAGAT1109450.12874878048695573No Hit
ACGCGCCTCCTCCTGTAGGCACCATCAATAGATCGG1087330.12618181214735372No Hit
TTTAGCCACGGAGCCTCTGTAGGCACCATCAATAGA1084930.1259032984034548No Hit
ACCCGTGGCGTGCGCCTGTAGGCACCATCAATAGAT1041840.12090281622653565No Hit
TTTTTACACCCGTGGCGTGCGCAATTCTGTAGGCAC1034350.12003362125078433No Hit
TCGTCCAGCGAATGAATGAAATTAGAACCAACGCTG1028600.11936634873935976No Hit
TATTCTATCACCCCCGGTCTGTAGGCACCATCAATA1023340.11875593945064788No Hit
ACCATAATCCACATCTCCGGCCCTGTAGGCACCATC1019370.1182952312992817No Hit
ATCCAACGGGAGGACTGTAGGCACCATCAATAGATC1013750.11764304494898499No Hit
AGATACAAGAAACACGGGAGCGGTTCCTCCCTGTAG1009650.11716725063649093No Hit
TACCCGTGGCGTGCGCCTGTAGGCACCATCAATAGA1004840.11660906267476011No Hit
AGATACAAGAAACCTGTAGGCACCATCAATAGATCG1003930.11650345954686511No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAG981950.11395273784232385Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTATCACCCCCGGTCTGTAGGCACCATCAATAG963140.11176988637451582No Hit
CCCCACTCTTGTCACCACTGTAGGCACCATCAATAG928620.10776393035810254No Hit
ATGATTCTCCCTTCTGTAGGCACCATCAATAGATCG879380.10204975671244233No Hit
ATCCTGCTGGTGACGCCCTGTAGGCACCATCAATAG878390.10193486979308401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)