FastQCFastQC Report
Wed 28 Jun 2023
SRR1272138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1272138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69724475
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGT66974569.605602623755862No Hit
ACGGGAGCGGTTCCTCCCTGTAGGCACCATCAATAG7414761.063437193324152No Hit
ACGGGAGCGGTTCCTCCTCTGTAGGCACCATCAATA6560390.9409020290220902No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4108520.5892507616586572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAGCGGTTCCTCTGTAGGCACCATCAATAGAT3441060.4935225399689277No Hit
ACGCGCCTCCTCATGCTGTAGGCACCATCAATAGAT3393510.4867028399998709No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACG3314900.47542846324766164No Hit
ACGTTTCTTCTGTAGGCACCATCAATAGATCGGAAG2508440.35976463071252957No Hit
ACGCGCCTCCTCCTGTAGGCACCATCAATAGATCGG2292450.32878698620534613No Hit
ACGCGCCTCCTCATCTGTAGGCACCATCAATAGATC1860100.26677863117649864No Hit
ACCCGTGGCGTGCGCAATTCTGTAGGCACCATCAAT1768960.2537071810149879No Hit
TCAGATACAAGAAACCTGTAGGCACCATCAATAGAT1618770.2321666817857001No Hit
GCCTCCTCATGCTGTAGGCACCATCAATAGATCGGA1547110.22188908557576087No Hit
GCGCCTCCTCATGCTGTAGGCACCATCAATAGATCG1424540.20430989261661703No Hit
CAGATACAAGAAACCTGTAGGCACCATCAATAGATC1372450.19683905830771764No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1302030.1867393049571187No Hit
AAGAAACACGGGAGCGGTTCCTCCTCTGTAGGCACC1239220.1777309904448904No Hit
ACGGAGCCTACGCGCCTCCTCATGCTGTAGGCACCA1167920.1675050260328242No Hit
TATCCTCAGCCTGTAGGCACCATCAATAGATCGGAA1140140.16352077229695886No Hit
ACGGGAGCGGTTCCTCCTGTAGGCACCATCAATAGA1136940.16306182298253233No Hit
AGCACGTTTCTTCTGTAGGCACCATCAATAGATCGG1065290.1527856609892007No Hit
TGCGCCTCCTCATGCTGTAGGCACCATCAATAGATC1015380.14562748590075436No Hit
GCGCCTCCTCATCTGTAGGCACCATCAATAGATCGG974960.1398303823729042No Hit
ATCACCCCCGGTCTGTAGGCACCATCAATAGATCGG949200.13613584039177062No Hit
AAGAAACACGGGAGCGGTTCCTCCCTGTAGGCACCA910440.13057681682077923No Hit
TACCCGTGGCGTGCGCAATTCTGTAGGCACCATCAA897700.1287496248627186No Hit
TTACGTGCCTATTCTATCCTGTAGGCACCATCAATA881250.1263903385432447No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGT834810.11972983661762959No Hit
TTTTACACCCGTGGCGTGCGCAATTCTGTAGGCACC828880.11887934616933295No Hit
ACCCCCGGTCTGTAGGCACCATCAATAGATCGGAAG807990.11588326767609221No Hit
CTGTAGGCGCCATCAATAGATCGGAAGAGCACACGT800580.11482051316987327No Hit
TTTAGCCACGGAGCCTCTGTAGGCACCATCAATAGA757890.1086978424721018No Hit
AGAGCATTCGGCTCTGTAGGCACCATCAATAGATCG733990.10527006477997862No Hit
TACGCGCCTCCTCATGCTGTAGGCACCATCAATAGA697720.10006816114427539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)