FastQCFastQC Report
Tue 5 Sept 2023
SRR12758953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70315497
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT7115191.0118950023207542No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC5476570.7788567575651212No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5052370.718528662323186No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC5025530.7147115805780339No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT4684120.6661575612556646No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC4090110.5816797398161034No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT4064770.5780759823115522No Hit
ACCTCTAGCATTACAAGTATTAGAGGC3849720.5474923970174028No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC3632790.5166414453417004No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC3223630.4584522811521904No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT3164050.44997904231552255No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT2767290.39355335851497997No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT2756710.39204871153794163No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2646700.3764035117322715No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2377840.33816727484696585No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC2354370.3348294615623637No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT2188380.31122300109746787No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2098510.29844203476226583No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT2060890.29309186280799526No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1908640.2714394523870037No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG1825600.25962982242733773No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1825040.2595501813775134No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1645810.23406077894891367No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1639290.2331335295831017No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT1577230.2243075946686404No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC1548500.2202217243803311No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1509980.21474355788169996No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA1473490.20955409018868204No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1460410.20769390281064215No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1405280.199853525887757No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1377590.19591556040626434No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC1374980.19554437622761878No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1246520.17727528826255753No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTT1242890.17675904360030337No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT1194160.16982885010398205No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT1176590.16733011216574348No Hit
AAAAGCATCTGGCCTACACCCAGAAGAT1140300.16216908770480568No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1130980.16084363308987207No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG1090880.15514076505780797No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1057370.1503751015227838No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC1055670.1501333340501028No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCC925560.13162958942038055No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC910730.1295205237616396No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC867130.12331989916817342No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAT866340.12320754840145694No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT862250.12262588430541849No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC860930.12243815897368968No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG854380.12150664312306574No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAC837510.1191074564971076No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC835720.11885288956999052No Hit
CCTCTAGCATTACAAGTATTAGAGGC822930.11703394487846683No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC806330.11467315661581685No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC792700.11273475034955666No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG780430.11098975806144128No Hit
CCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC764260.10869012274776356No Hit
AGTTCAATTTTAAACTTGCTAAAAAAAC757180.1076832323321273No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC753110.10710441255929684No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC737130.10483179831609524No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCC736120.10468815999409063No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC732150.10412356183730025No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC708480.10075730532061802No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC703390.10003342506417895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)