FastQCFastQC Report
Tue 5 Sept 2023
SRR12758954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32803611
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2456710.7489145021260006No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2064120.6292356045802396No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1667660.5083769588659004No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT1493470.45527609750036363No Hit
ATGTACACGGAGTCGACCCAACGCGA1309660.39924263216022166No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1051170.32044338045588944No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC960500.2928031307285043No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC926450.28242317591194455No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT912770.2782529033160404No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG909640.27729874006858574No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC899240.27412835739333696No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC885320.26988492212031173No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG864450.26352281765565383No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG775350.23636117377443597No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC771090.23506253625553603No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA745460.22724937202797582No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC678390.20680345221750132No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT660840.20145343145301897No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT620600.1891864892557103No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC617140.1881317273272141No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC616280.18786956106753003No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT610320.18605268791902208No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCT586110.17867240286442854No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC570270.17384366617443428No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT562030.17133174759327563No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT558820.17035319678678057No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC557460.1699386082830942No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC550900.16793882844178343No Hit
ACCTCTAGCATTACAAGTATTAGAGGC535920.16337225801147318No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC520920.15879959069140284No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCC506690.15446165362709613No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC503820.15358674994652266No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC487440.14859339723300585No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC469170.14302388843716016No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT461250.14060952009216302No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC457310.13940843280942455No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGC455880.13897250519157783No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT453370.13820734552668607No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG452170.13784153214108044No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGT446740.13618622657121499No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT444970.13564665182744667No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGC441590.13461627745799085No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC440530.13429314230070585No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC426800.13010762748040147No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGAC420590.12821454320989234No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT417330.12722075017899706No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC405990.1237638136850239No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC402950.12283708644148963No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC397090.12105069774178215No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT396480.12086474260409928No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT375980.11461543060000315No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC374110.11404537140743437No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT370440.11292659213645716No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC368560.11235348449900835No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCT365920.11154869505067598No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG356550.10869230219807202No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC348950.10637548408923639No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC346940.10576274666834697No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT346830.10572921377466646No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCT337640.10292769292990335No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC332150.1012540966907576No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT331950.10119312779315666No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT330320.10069623127770903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)