FastQCFastQC Report
Tue 5 Sept 2023
SRR12758955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40650035
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAAGCATCTGGCCTACACCCAGAAGATT2439140.6000339237100287No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2204590.5423340963913069No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT2127310.5233230426492869No Hit
ACCTCTAGCATTACAAGTATTAGAGGC1879260.4623021849796685No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1533780.37731332826650704No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1368650.33669097701883893No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1234560.30370453555575044No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1222960.3008509094764617No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC1155160.28417195704751547No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1133060.278735307361974No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG1115040.27430234684914784No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT1099080.27037615096764367No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGC1090380.26823593140817714No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC973440.2394684285019681No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT956660.235340510776928No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC930410.22888295176129617No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC869750.21396045538460173No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT855500.21045492334754445No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT837770.20609330348670055No Hit
ATGTACACGGAGTCGACCCAACGCGA772590.1900588769480764No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC750580.18464436746487425No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT729900.17955704097179745No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC698460.1718227302879321No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT676760.16648448150167644No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT660340.1624451245859936No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT659910.16233934361926133No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC656870.16159149678468912No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC636800.15665423166302317No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC612310.15062963660424894No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC611370.15039839449092726No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG610730.15024095305206994No Hit
CCTCTAGCATTACAAGTATTAGAGGC590790.1453356682226719No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT584370.1437563337891345No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT581280.14299618684215154No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCG575330.141532473465275No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC568440.13983751797507676No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC563250.13856076630684327No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC551870.13576126072216174No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT550280.1353701171475006No Hit
AGAGCCACCTTACAAATAAGCGCTCTC548350.13489533280844654No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG547170.13460505015555338No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT547170.13460505015555338No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG539500.13271821291174782No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCC539140.13262965210239058No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC532080.13089287622999587No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC523720.12883629743492225No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT523240.12871821635577926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC506320.12455585831598916No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC505870.12444515730429259No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC504980.12422621530338165No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC494880.12174159259641475No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC489000.1202950993769132No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT485760.11949805209269808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG469410.11547591533439025No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGC469110.11540211465992589No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC454930.11191380278024361No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG444620.10937751960115165No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAC440900.10846239123779353No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC438540.1078818259320072No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT435600.10715857932225643No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC424700.10447715481671786No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC424420.1044082741872178No Hit
ACCTCTAGCATTACAAGTATTAGAGGCACT412480.10147100734353612No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGT411010.10110938403866074No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT408690.100538658822803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)