FastQCFastQC Report
Tue 5 Sept 2023
SRR12758956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22720115
Total Bases718.8 Mbp
Sequences flagged as poor quality0
Sequence length1-60
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1954240.8601364913865973No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1756150.7729494326943327No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1740850.7662153118503141No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1641970.7226944053760291No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT1421820.6257978887870946No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1077510.4742537614796404No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC1044630.4597820037442592No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC850580.3743731050657094No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT761760.3352799930810209No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC727330.32012602048889277No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC706200.310825891506271No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT702160.30904773149255627No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT647230.2848709172466777No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC610360.2686430064284446No Hit
ACCTCTAGCATTACAAGTATTAGAGGC596390.2624942699453766No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC544330.23958065353102304No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC519650.22871803245714206No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACAC513550.2260331868918797No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT502580.2212048662605801No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC494900.2178246016800531No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC482000.21214681351744918No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT461190.20298752889234936No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC458260.20169792274378892No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC448030.1971953046892588No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC444470.19562841121182703No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGAC443740.19530711002123008No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC438460.19298317812211777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG438340.19293036148804704No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC422480.185949763018365No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT421850.18567247568949363No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG412370.18149996159790566No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT397860.17511355026151937No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT396060.17432130075045835No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT390360.1718125106320985No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT381160.16776323535334217No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC371530.16352470046916576No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGC363440.15996397905556375No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC358510.15779409567249109No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT352840.15529850971264889No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT350250.15415855069395554No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC350090.15408812851519457No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC349100.153652391284111No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC347370.15289095147625792No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAA336240.14799220866619733No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT333520.14679503162726068No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC332010.14613042231520396No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT331990.14612161954285882No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG330440.14543940468611183No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC329280.14492884389009475No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC322560.14197111238213364No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT315430.1388329240410975No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC312980.13775458442882002No Hit
CCCCGGATAGCTCAGTCGGTAGAGCATCAGAC297210.130813598434691No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC297010.1307255707112398No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC295720.1301577918949794No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC292740.12884617881555618No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT291140.1281419570279464No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGAC288380.1269271744443195No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG282420.12430394828547302No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC281810.12403546372894679No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC279530.12303194768160285No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT278550.12260061183669184No Hit
GGAGGCGTGGGTTCGAATCCCACTTCTGACAC274320.12073882548569848No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCG269290.1185249282409002No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC266460.11727933595406537No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC258550.11379783949156948No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC255660.11252583888769929No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT254220.11189203927885048No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG252110.11096334679644008No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA251010.11047919431745835No Hit
GGAGGCGTGGGTTCGGATCCCACTTCTGACAC249830.10995983074909611No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG247940.10912796876248204No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT247520.10894311054323448No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCC239910.10559365566591543No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC239020.10520193229655748No Hit
TGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC235130.10348979307543119No Hit
TTTCCATAGTGTAGCGGTTATCACGTCTGCT233020.10256110059302076No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGC232920.10251708673129514No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC228610.10062008929092128No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC227640.10019315483218284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)