FastQCFastQC Report
Tue 5 Sept 2023
SRR12758957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103596157
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG15823461.527417662799982No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT13935201.3451464227577477No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT9017560.8704531385271367No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT8902670.8593629588016474No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT7558620.7296235901878098No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC6745560.6511399838895569No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC5564930.5371753317065613No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT4786520.4620364440738859No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG4731640.4567389502682035No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC4608080.4448118669112407No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC4358070.4206787323201574No Hit
ACCTCTAGCATTACAAGTATTAGAGGC4027950.3888126853972006No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC3931620.3795140779208634No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG3901900.3766452456339669No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCG3768900.36380693156407334No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC3695830.35675358111980926No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT3489670.33685322902470216No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT3450980.3331185345031669No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT3242990.3130415349287522No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT3215920.31042850363648145No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC3065080.2958681179650322No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG2971000.28678670001243384No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2968010.2864980792675543No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC2812570.27149366168090583No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT2797140.2700042241914437No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC2769890.26737381773727376No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT2667640.2575037604918105No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC2662150.25697381805388786No Hit
TTTCCATAGTGTAGCGGTTATCACGTCTGCT2658850.2566552734190709No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAT2652380.2560307328774754No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGC2618280.25273910498436736No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGACAC2535210.24472046776793083No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2506730.2419713310407837No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT2502140.24152826441235653No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTT2447970.2362993059674984No Hit
CCGCCGCTGGTGTAGTGGTATCATGCAAGATT2429300.23449711556385242No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2288340.22089043322330965No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC2285430.22060953477260745No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC2212850.21360348338017981No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT2181050.21053387144467145No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC2160510.20855117241462923No Hit
TGCCGCTGGTGTAGTGGTATCATGCAAGATT2113330.20399694942351965No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2107360.2034206732205327No Hit
GCATTGGTGGTTCAGTGG2096590.20238105936690298No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGAT2082200.20099201170174683No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT1968260.18999353421961396No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC1903790.1837703303994182No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGA1831180.17676138314667406No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1776260.171460028193903No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCG1729050.16690290934247687No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATT1714130.1654627014783956No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC1684760.1626276542285251No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC1656030.15985438533207366No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1654310.15968835600726No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1633890.15771724041848387No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCC1619420.15632047045915032No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAA1585120.15300953683059884No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC1582300.15273732596084622No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1578980.15241685075248496No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1576330.15216104975785927No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCG1558410.15043125586212625No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC1558240.1504148459869993No Hit
AATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1539710.14862616959816374No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT1538610.1485199880532248No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1534130.14808753957929155No Hit
GCGAAAGGTCCCCGGTTCGTAACCGGGCGGAAA1514320.14617530648361793No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT1505980.1453702573156261No Hit
CCCCGGATAGCTCAGTCGGTAGAGCATCAGAC1494970.14430747657946424No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC1446980.13967506535980867No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1422180.137281154164821No Hit
ATTCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG1397510.13489979169787158No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTC1395030.1346604005783728No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATT1373870.13261785376845592No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1355360.13083110795316472No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCT1328960.12828275087462945No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC1328010.1281910486312731No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1322450.12765434918594518No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGC1319050.12732615168340655No Hit
GGAGGCGTGGGTTCGAATCCCACTTCTGACAC1241950.119883790669957No Hit
GGAGGCGTGGGTTCGGATCCCACTTCTGACAC1226370.11837987387891231No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGG1217660.11753910910035012No Hit
TAGAGTGGCGCAGCGGAAGCGTGCTGGGC1216730.11744933743053809No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1205070.11632381305418502No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1192290.11509017655934864No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAAT1186830.11456312998174246No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTT1179600.11386522764546178No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGC1178680.11377642126242193No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1168550.11279858576221125No Hit
CCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC1153840.11137864892034556No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC1152940.11129177311085005No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGAC1147230.11074059436393958No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT1138360.10988438499702263No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGC1130660.10914111418244983No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1130630.10913821832213332No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1126600.10874920775294783No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1124700.10856580326623506No Hit
TTCCCGGGGAGCCCGGCGGGTGCCGGCGCGGG1124330.10853008765566467No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG1119560.10806964586533843No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1116950.10781770601780141No Hit
GGAGGCGTGGGTTCGTATCCCACTTCTGA1106580.10681670363505857No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1095590.10575585347244107No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG1088010.10502416609913437No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATCAGAC1087050.1049314985690058No Hit
CGAAAGGTCCCCGGTTCGTAACCGGGCGGAAA1072720.10354824262448269No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAA1070170.1033020944975787No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCT1069790.10326541360023615No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG1065760.10287640303105067No Hit
ATTGGTGGTTCAGTGG1065250.10282717340566985No Hit
ATTCTTGCGACCCGGGTTCGATTCCCGGGCGGCG1059460.10226827236458201No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAA1044550.10082902978727291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)