FastQCFastQC Report
Tue 5 Sept 2023
SRR12758958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69889486
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC6798260.9727156957485708No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT6771570.9688968094571478No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC6420420.9186532005686806No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5951170.8515114848605411No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT5850210.8370658213168144No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT3490220.4993912818302884No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC3350420.47938827308015974No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT3294180.4713412830078619No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT3174620.4542342749523154No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT2548580.36465856967384197No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT2537610.36308894874402137No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC2479640.35479442501551667No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT2460170.35200859826040215No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT2346180.3356985627280189No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC2205700.31559825751186665No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC2089790.2990135025460053No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1969410.28178916639907753No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1926440.27564088824462096No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1870330.26761249896729816No Hit
ACCTCTAGCATTACAAGTATTAGAGGC1803630.2580688603146974No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1781220.25486236942706947No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1774480.2538979897491305No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC1751850.25066002059308323No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT1747100.24998037616130128No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1672460.23930065818483767No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG1575890.22548312917911573No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1542140.22065407663750738No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1472690.21071695962966447No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1358320.1943525525427387No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1342940.192151935414148No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTT1294430.18521097722767627No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT1212660.17351107718834846No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC1179740.16880078356850414No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG1173480.1679050837489347No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT1172990.16783497306018247No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC1132040.16197572264303103No Hit
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT1097720.15706511276960886No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC1095850.1567975474880442No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1054980.15094974371395434No Hit
TATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1040770.14891653374013938No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG1028760.14719810645051817No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC1010550.14459256432362372No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT990130.14167080868215284No Hit
TTTCCATAGTGTAGCGGTTATCACGTCTGCT971980.13907385153755458No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC971200.13896224676770408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG957030.13693476011541994No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC952650.1363080564077979No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCG950460.13599470455398685No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG947800.13561410367218898No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT925630.13244195271374581No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCC921750.1318867905252587No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT911720.13045166765141183No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG891040.12749271041999077No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG841030.12033712767611426No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC834680.11942855038310053No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC830970.1188977123111193No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGC823050.11776449464802187No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC821140.11749120604492641No Hit
ACCTCTAGCATTACAAGTATTAGAGGCAC779550.11154038248328225No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC764270.10935407365851853No Hit
AAAAGCATCTGGCCTACACCCAGAAGAT752090.10761132225239142No Hit
AATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG746250.10677571730889537No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT741570.10610608868979234No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGT737790.10556523480513222No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAA734800.10513741652070528No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC720690.10311851485071731No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT718990.10287527368565852No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCC703910.10071758146854878No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC703060.10059596088601938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)