FastQCFastQC Report
Tue 5 Sept 2023
SRR12758960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12758960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43630321
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length1-60
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC3273840.7503589075129655No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT3219820.7379776096536168No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC2331040.5342706509081151No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT2320260.5317998920979747No Hit
ACCTCTAGCATTACAAGTATTAGAGGC1750260.4011568010237651No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1675450.38401046831628854No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1613790.3698780946397346No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC1479560.3391127926837852No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1424280.3264427048336408No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC1292910.2963329103171164No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1187100.272081427042446No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1137820.2607865296246617No Hit
TAACCTAGACTTACAAGTCAAAGTAAAAT1125580.2579811411426471No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATTT1122270.2572224944207951No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC1032820.23672069705835994No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCC921160.21112840311213849No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC886950.20328752566363192No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGG876980.20100241756186027No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT873660.20024147885595434No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGG865180.19829787637821872No Hit
AACCTAGACTTACAAGTCAAAGTAAAAT864360.19810993368579616No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT854720.1959004610578043No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAC800770.18353520708683302No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGC780250.1788320558081615No Hit
AAAAGCATCTGGCCTACACCCAGAAGATT770450.17658591143530666No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC761350.17450020594622717No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGAC755890.1732487826527795No Hit
GCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC737030.16892610072706088No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT732130.16780302854063348No Hit
CCTCTAGCATTACAAGTATTAGAGGC730890.1675188225179457No Hit
AGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC723480.165820462333981No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC721310.16532310179427742No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC672720.15418635127621452No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT663080.15197687864822265No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCG656540.15047792107695013No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTC653430.14976511403617682No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT648720.14868558954677413No Hit
TTCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCT643240.14742958228521857No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGAT638860.1464256932695957No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT624650.14316878392895618No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAC619310.1419448644441557No Hit
CGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCAC618010.14164690651714437No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC604910.13864440740649148No Hit
AAGTTCAATTTTAAACTTGCTAAAAAAAC598570.1371912895162976No Hit
TTTTCCGTAGTGTAGTGGTTATCACGTTCGCC573640.1314773732698414No Hit
AGAGTTGCGCAGCGGAAGCGTGCTGGGC573180.13137194200336044No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT571890.13107627606040304No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC559440.12822275591325583No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGC539940.12375338700808551No Hit
ATTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC524910.12030853497502345No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG517120.11852307939700926No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGC516540.11839014432188111No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG512510.11746647474814591No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCC512180.1173908392743661No Hit
AGAGCCACCTTACAAATAAGCGCTCTC492400.11285729481568564No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT485920.11137208914873674No Hit
TGCGCCGCTGGTGTAGTGGTATCATGCAAGATT479440.10988688348178781No Hit
TGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT467620.10717775833003843No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC459560.105330419182568No Hit
TCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT457430.10484222657908017No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAAC454540.10417984318749339No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC450520.10325846559781213No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCG449970.10313240647484578No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC444810.10194974270301609No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC436620.10007260776284455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)