FastQCFastQC Report
Tue 5 Sept 2023
SRR12763765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12763765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48245619
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGG877904818.196570345589304No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCG5781631.1983740948582295No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCAG4235190.8778392914805384No Hit
CGCGGGATAGAGCAG3712510.7695019935385221No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGC3216350.6666615677581005No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCG3142370.6513275329724757No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGG3113840.6454140426719367No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGC2365120.4902248222786819No Hit
GCGGGATAGAGCAGTCTGGTAGCTCGTCGG2288910.4744285693588054No Hit
AGAGCAGTCTGGTAGCTCGTCGG2193620.45467755320954634No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCGC2002950.4151568663674934No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGGCGG1996610.41384275741181803No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1911890.39628261376437096TruSeq Adapter, Index 6 (100% over 49bp)
GAGGTTGTAGCTCAGACGGATAGAGCACTTG1407810.29180058815288495No Hit
TGGTCTGTAGTTCAATTGGTTAGAGCACTTG1336110.276939135136809No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCT1277560.2648033182038767No Hit
GGCGGGATAGAGCAGTCTGGTAGCTCGTCGG1225090.2539277193230747No Hit
TCTGGTAGCTCGTCGG1199590.24864226532154143No Hit
CGGGATAGAGCAGTCTGGTAGCTCGTCGG1070470.22187921353024823No Hit
CGCGGGATAGAGCAGT978110.20273550640940058No Hit
CCCGAAGGTCGGTGGTTCAAATCCGCCTCCCG972100.2014897974466863No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGT956660.1982895068669344No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTC911430.18891456237715595No Hit
CGCGGGATAGAGCAGTCTGG882690.18295754480836904No Hit
CGCGGGATAGAGCACTTG870000.18032725416995893No Hit
CTGGTAGCTCGTCGG844420.17502521835195026No Hit
AGCAGGATGTCAGCGGTTCGAGCCCGCTTAGCTC821420.17025794611527317No Hit
AGTCTGGTAGCTCGTCGG786530.16302620140494No Hit
CGCGGGATAGAGCAGTCTG756750.15685362022197288No Hit
GGACACGTAGCTCAGTGGATAGAGCACTTG742440.153887547799936No Hit
CGCGGGATAGAGCAGTCTGGTAG734570.15225631160416866No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCCTG709520.1470641303203095No Hit
ATAGAGCAGTCTGGTAGCTCGTCGG694390.14392809427939973No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCC652900.1353283497098462No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCACTTG633260.13125751376513584No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTAGG616540.12779191412177757No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCGCACC608370.1260984961142275No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCCG553550.11473580637446067No Hit
CGCGGGATAGCGCAGTCTGGTAGCTCGTCGG549660.1139295155483444No Hit
AGCAGGATGTCGGCGGTTCAAGTCCGCCTCCTGG540270.1119832248395445No Hit
CGCGGGATAGAGCAGTCT528900.10962653417297848No Hit
CCGCGGGATAGAGCAGTCTGGTAGCTCGTCGG518260.10742115258175049No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGCCGG503940.10445300743265414No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCC502770.10421049836670145No Hit
ACGCGGGATAGAGCAGTCTGGTAGCTCGTCGG494100.10241344400618013No Hit
CGCGGTATAGAGCAGTCTGGTAGCTCGTCGG489490.10145791683178527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)