FastQCFastQC Report
Tue 5 Sept 2023
SRR12763772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12763772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40009379
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGG542101213.5493530154517No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCT12246713.060959781455243No Hit
AGAGTAGAAGTCTTTCAAACTATTGCCTAGGCGAGG5110481.2773205002756978No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGC4411091.102513988032656No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2535720.6337813941076166TruSeq Adapter, Index 9 (100% over 49bp)
GCGGGATAGAGCAGTCTGGTAGCTCGTCGG2426590.6065052896722041No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCTC2149500.5372490285340344No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCG1836060.4589073976879271No Hit
AGAGTAGAAGTCTTTCAAACTATTGCCTAGGCGAG1487880.3718828027798182No Hit
CATCCTGGAACAGAACCCGGCTTACGACTAACTCTC1303430.3257811124736527No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCGCCAC1296420.32402902329476296No Hit
CGCGGGATAGAGCAGTCTGG1195580.29882493302382923No Hit
ACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCGC1130400.28253375289828914No Hit
AGTCTGGTAGCTCGTCGGGCTCATAACC938660.23460998982263634No Hit
AGAGTAGAAGTCTTTCAAACTATTGCCTAGGC896410.2240499658842493No Hit
TCCCCATCGTCTAGCGGCCTAGGACACTGC895100.22372254265681052No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTCGGG775930.1939370266156843No Hit
AGAGTAGAAGTCTTTCAAACTATTGCCTAGGCG761560.1903453687696577No Hit
GAGTAGAAGTCTTTCAAACTATTGCCTAGGCGAGG739240.18476667683344947No Hit
CGCGGGATAGAGCAGTCTGGTAGCTCGTC731170.1827496497758688No Hit
CGGGATAGAGCAGTCTGGTAGCTCGTCGG670680.16763069479283846No Hit
CCCGAAGGTCGGTGGTTCAAATCCGCCTCCCGCCAC670010.16746323405819422No Hit
AAATCTTGTCAGGACCGGAAGGTAGCAG623030.15572098732149778No Hit
ATCCTGGAACAGAACCCGGCTTACGACTAACTCTC587470.14683307131560328No Hit
AACCCGAAGGTCGGTGGTTCAAATCCGCCTCCCGCCAC581020.1452209493179087No Hit
AGGGTGAGGTCTCTGGTTCAAGTCCAGAATGGCCCGCCCCCATCGTCTAG557280.13928734060081263No Hit
AAGGGATGCTTGTGGTAGGCGTGAACTCCGGGC553130.13825008381159826No Hit
GCGGGATAGAGCAGTCTGGTAGCTCGTCGGGCT541700.13539325366684646No Hit
AGAGCAGTCTGGTAGCTCGTCGG540840.13517830406715384No Hit
ACGCCCAAATCTTGTCAGGACCGGAAGGTAGCAG538220.13452345761227638No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC533770.13341121840456457TruSeq Adapter, Index 9 (100% over 50bp)
AGTAAGATTTAAGGAAACCAAAACTATGCAAGACG418880.10469545153400156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)